Air-water interface displaces adsorbed bacteria

Video microscopy was employed to observed the spatial distribution of Staphylococcus epidermidis and Pseudomonas aeruginosa adherent to glass and polymer substrates. During rinsing procedures the bacteria remained in their original positions when the surfaces were rinsed with saline for 3 min follow...

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Bibliographic Details
Published in:Biomaterials 1993, Vol.14 (8), p.605-608
Main Authors: Pitt, William G., McBride, Michael O., Barton, Alan J., Sagers, Richard D.
Format: Article
Language:English
Subjects:
Adsorption
Air
Bacteria
Bacterial Adhesion
Biological and medical sciences
Cell interactions, adhesion
Ethanol
Fundamental and applied biological sciences. Psychology
Glass
Molecular and cellular biology
polymer surfaces
Polymers
Pseudomonas aeruginosa - physiology
rinsing
Staphylococcus epidermidis - physiology
Surface Properties
Time Factors
Water - chemistry
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Summary:Video microscopy was employed to observed the spatial distribution of Staphylococcus epidermidis and Pseudomonas aeruginosa adherent to glass and polymer substrates. During rinsing procedures the bacteria remained in their original positions when the surfaces were rinsed with saline for 3 min followed by ethanol for 3 min before exposure to air. When the surfaces were rinsed with saline only, the air-liquid interface disrupted the spatial distribution of the bacteria, removing and redepositing the bacteria in clumps. A moving air-liquid interface of a gas bubble on substrate also displaced bacteria. Such artefacts produced by air-water interfaces should be avoided during bacterial adhesion experiments.
ISSN:0142-9612
1878-5905
DOI:10.1016/0142-9612(93)90179-6