Loading…
Air-water interface displaces adsorbed bacteria
Video microscopy was employed to observed the spatial distribution of Staphylococcus epidermidis and Pseudomonas aeruginosa adherent to glass and polymer substrates. During rinsing procedures the bacteria remained in their original positions when the surfaces were rinsed with saline for 3 min follow...
Saved in:
Published in: | Biomaterials 1993, Vol.14 (8), p.605-608 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c418t-8e82641cedef4a0d4d08d9d876c170b29101d91d998e6b952574c102427826db3 |
---|---|
cites | cdi_FETCH-LOGICAL-c418t-8e82641cedef4a0d4d08d9d876c170b29101d91d998e6b952574c102427826db3 |
container_end_page | 608 |
container_issue | 8 |
container_start_page | 605 |
container_title | Biomaterials |
container_volume | 14 |
creator | Pitt, William G. McBride, Michael O. Barton, Alan J. Sagers, Richard D. |
description | Video microscopy was employed to observed the spatial distribution of
Staphylococcus epidermidis and
Pseudomonas aeruginosa adherent to glass and polymer substrates. During rinsing procedures the bacteria remained in their original positions when the surfaces were rinsed with saline for 3 min followed by ethanol for 3 min before exposure to air. When the surfaces were rinsed with saline only, the air-liquid interface disrupted the spatial distribution of the bacteria, removing and redepositing the bacteria in clumps. A moving air-liquid interface of a gas bubble on substrate also displaced bacteria. Such artefacts produced by air-water interfaces should be avoided during bacterial adhesion experiments. |
doi_str_mv | 10.1016/0142-9612(93)90179-6 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75985294</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0142961293901796</els_id><sourcerecordid>75985294</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-8e82641cedef4a0d4d08d9d876c170b29101d91d998e6b952574c102427826db3</originalsourceid><addsrcrecordid>eNp9kMtKAzEUhoMotVbfQKEL8bIYm6TJJGcjlOINCm50HTLJGYhMZ2rSKr69qR26FEIunO8_ST5Czhm9Y5SVE8oEL6Bk_Aamt0CZgqI8IEOmlS4kUHlIhnvkmJyk9EHzmQo-IAM9BQAphmQyC7H4tmuM49DmubYOxz6kVZM3aWx96mKFflxZl6vBnpKj2jYJz_p1RN4fH97mz8Xi9ellPlsUTjC9LjRqXgrm0GMtLPXCU-3Ba1U6pmjFIf_AQx6gsaxAcqmEY5QLrnLQV9MRudr1XcXuc4NpbZYhOWwa22K3SUZJ0JKDyOD1_6CQpVSUqkyKHelil1LE2qxiWNr4Yxg1W6Nmq8tsdRmYmj-jpsyxi_6CTbVEvw_1CnP9sq_b5GxTR9u6kPaYUACU84zd7zDM1r4CRpNcwDYbChHd2vgu_P-OX7Lkj2I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>745657007</pqid></control><display><type>article</type><title>Air-water interface displaces adsorbed bacteria</title><source>ScienceDirect: Materials Science Backfile</source><creator>Pitt, William G. ; McBride, Michael O. ; Barton, Alan J. ; Sagers, Richard D.</creator><creatorcontrib>Pitt, William G. ; McBride, Michael O. ; Barton, Alan J. ; Sagers, Richard D.</creatorcontrib><description>Video microscopy was employed to observed the spatial distribution of
Staphylococcus epidermidis and
Pseudomonas aeruginosa adherent to glass and polymer substrates. During rinsing procedures the bacteria remained in their original positions when the surfaces were rinsed with saline for 3 min followed by ethanol for 3 min before exposure to air. When the surfaces were rinsed with saline only, the air-liquid interface disrupted the spatial distribution of the bacteria, removing and redepositing the bacteria in clumps. A moving air-liquid interface of a gas bubble on substrate also displaced bacteria. Such artefacts produced by air-water interfaces should be avoided during bacterial adhesion experiments.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/0142-9612(93)90179-6</identifier><identifier>PMID: 8399954</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adsorption ; Air ; Bacteria ; Bacterial Adhesion ; Biological and medical sciences ; Cell interactions, adhesion ; Ethanol ; Fundamental and applied biological sciences. Psychology ; Glass ; Molecular and cellular biology ; polymer surfaces ; Polymers ; Pseudomonas aeruginosa - physiology ; rinsing ; Staphylococcus epidermidis - physiology ; Surface Properties ; Time Factors ; Water - chemistry</subject><ispartof>Biomaterials, 1993, Vol.14 (8), p.605-608</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-8e82641cedef4a0d4d08d9d876c170b29101d91d998e6b952574c102427826db3</citedby><cites>FETCH-LOGICAL-c418t-8e82641cedef4a0d4d08d9d876c170b29101d91d998e6b952574c102427826db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0142961293901796$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3555,4024,27923,27924,27925,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4799022$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8399954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pitt, William G.</creatorcontrib><creatorcontrib>McBride, Michael O.</creatorcontrib><creatorcontrib>Barton, Alan J.</creatorcontrib><creatorcontrib>Sagers, Richard D.</creatorcontrib><title>Air-water interface displaces adsorbed bacteria</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Video microscopy was employed to observed the spatial distribution of
Staphylococcus epidermidis and
Pseudomonas aeruginosa adherent to glass and polymer substrates. During rinsing procedures the bacteria remained in their original positions when the surfaces were rinsed with saline for 3 min followed by ethanol for 3 min before exposure to air. When the surfaces were rinsed with saline only, the air-liquid interface disrupted the spatial distribution of the bacteria, removing and redepositing the bacteria in clumps. A moving air-liquid interface of a gas bubble on substrate also displaced bacteria. Such artefacts produced by air-water interfaces should be avoided during bacterial adhesion experiments.</description><subject>Adsorption</subject><subject>Air</subject><subject>Bacteria</subject><subject>Bacterial Adhesion</subject><subject>Biological and medical sciences</subject><subject>Cell interactions, adhesion</subject><subject>Ethanol</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glass</subject><subject>Molecular and cellular biology</subject><subject>polymer surfaces</subject><subject>Polymers</subject><subject>Pseudomonas aeruginosa - physiology</subject><subject>rinsing</subject><subject>Staphylococcus epidermidis - physiology</subject><subject>Surface Properties</subject><subject>Time Factors</subject><subject>Water - chemistry</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNp9kMtKAzEUhoMotVbfQKEL8bIYm6TJJGcjlOINCm50HTLJGYhMZ2rSKr69qR26FEIunO8_ST5Czhm9Y5SVE8oEL6Bk_Aamt0CZgqI8IEOmlS4kUHlIhnvkmJyk9EHzmQo-IAM9BQAphmQyC7H4tmuM49DmubYOxz6kVZM3aWx96mKFflxZl6vBnpKj2jYJz_p1RN4fH97mz8Xi9ellPlsUTjC9LjRqXgrm0GMtLPXCU-3Ba1U6pmjFIf_AQx6gsaxAcqmEY5QLrnLQV9MRudr1XcXuc4NpbZYhOWwa22K3SUZJ0JKDyOD1_6CQpVSUqkyKHelil1LE2qxiWNr4Yxg1W6Nmq8tsdRmYmj-jpsyxi_6CTbVEvw_1CnP9sq_b5GxTR9u6kPaYUACU84zd7zDM1r4CRpNcwDYbChHd2vgu_P-OX7Lkj2I</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Pitt, William G.</creator><creator>McBride, Michael O.</creator><creator>Barton, Alan J.</creator><creator>Sagers, Richard D.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Air-water interface displaces adsorbed bacteria</title><author>Pitt, William G. ; McBride, Michael O. ; Barton, Alan J. ; Sagers, Richard D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-8e82641cedef4a0d4d08d9d876c170b29101d91d998e6b952574c102427826db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adsorption</topic><topic>Air</topic><topic>Bacteria</topic><topic>Bacterial Adhesion</topic><topic>Biological and medical sciences</topic><topic>Cell interactions, adhesion</topic><topic>Ethanol</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glass</topic><topic>Molecular and cellular biology</topic><topic>polymer surfaces</topic><topic>Polymers</topic><topic>Pseudomonas aeruginosa - physiology</topic><topic>rinsing</topic><topic>Staphylococcus epidermidis - physiology</topic><topic>Surface Properties</topic><topic>Time Factors</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pitt, William G.</creatorcontrib><creatorcontrib>McBride, Michael O.</creatorcontrib><creatorcontrib>Barton, Alan J.</creatorcontrib><creatorcontrib>Sagers, Richard D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pitt, William G.</au><au>McBride, Michael O.</au><au>Barton, Alan J.</au><au>Sagers, Richard D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Air-water interface displaces adsorbed bacteria</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>1993</date><risdate>1993</risdate><volume>14</volume><issue>8</issue><spage>605</spage><epage>608</epage><pages>605-608</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Video microscopy was employed to observed the spatial distribution of
Staphylococcus epidermidis and
Pseudomonas aeruginosa adherent to glass and polymer substrates. During rinsing procedures the bacteria remained in their original positions when the surfaces were rinsed with saline for 3 min followed by ethanol for 3 min before exposure to air. When the surfaces were rinsed with saline only, the air-liquid interface disrupted the spatial distribution of the bacteria, removing and redepositing the bacteria in clumps. A moving air-liquid interface of a gas bubble on substrate also displaced bacteria. Such artefacts produced by air-water interfaces should be avoided during bacterial adhesion experiments.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8399954</pmid><doi>10.1016/0142-9612(93)90179-6</doi><tpages>4</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0142-9612 |
ispartof | Biomaterials, 1993, Vol.14 (8), p.605-608 |
issn | 0142-9612 1878-5905 |
language | eng |
recordid | cdi_proquest_miscellaneous_75985294 |
source | ScienceDirect: Materials Science Backfile |
subjects | Adsorption Air Bacteria Bacterial Adhesion Biological and medical sciences Cell interactions, adhesion Ethanol Fundamental and applied biological sciences. Psychology Glass Molecular and cellular biology polymer surfaces Polymers Pseudomonas aeruginosa - physiology rinsing Staphylococcus epidermidis - physiology Surface Properties Time Factors Water - chemistry |
title | Air-water interface displaces adsorbed bacteria |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T14%3A11%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Air-water%20interface%20displaces%20adsorbed%20bacteria&rft.jtitle=Biomaterials&rft.au=Pitt,%20William%20G.&rft.date=1993&rft.volume=14&rft.issue=8&rft.spage=605&rft.epage=608&rft.pages=605-608&rft.issn=0142-9612&rft.eissn=1878-5905&rft_id=info:doi/10.1016/0142-9612(93)90179-6&rft_dat=%3Cproquest_cross%3E75985294%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c418t-8e82641cedef4a0d4d08d9d876c170b29101d91d998e6b952574c102427826db3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=745657007&rft_id=info:pmid/8399954&rfr_iscdi=true |