Studies on the pathophysiological mechanism of Campylobacter jejuni‐induced fluid secretion in rat ileum

Calcium has been reported to play an important role in regulating the intestinal electrolyte transport via Ca2+/calmodulin (CaM) and/or protein kinase C (PKC) systems. The role of Ca2+, CaM and PKC in the pathogenesis of Campylobacter jejuni‐induced fluid accumulation was studied in vivo in ligated...

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Bibliographic Details
Published in:FEMS microbiology letters 1993-08, Vol.111 (2‐3), p.327-330
Main Authors: Kaur, Rupinder, Ganguly, N.K., Kumar, Lata, Walia, B.N.S.
Format: Article
Language:English
Subjects:
Animals
Bacteriology
Biological and medical sciences
Body Fluids - secretion
Calcium - metabolism
Calcium ion
Calmodulin
Calmodulin - metabolism
Campylobacter Infections - etiology
Campylobacter Infections - physiopathology
Campylobacter jejuni
Campylobacter jejuni - pathogenicity
Diarrhea - etiology
Diarrhea - physiopathology
Fundamental and applied biological sciences. Psychology
Humans
Ileum - secretion
Intestinal fluid accumulation
Male
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Protein kinase C
Protein Kinase C - metabolism
Rats
Rats, Wistar
Second Messenger Systems - physiology
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Summary:Calcium has been reported to play an important role in regulating the intestinal electrolyte transport via Ca2+/calmodulin (CaM) and/or protein kinase C (PKC) systems. The role of Ca2+, CaM and PKC in the pathogenesis of Campylobacter jejuni‐induced fluid accumulation was studied in vivo in ligated rat ileal loops. Calcium ionophore A23187 (5 μM) and PKC activator, phorbol‐12‐myristate‐13‐acetate (PMA, 100 μg kg−1) when injected alone induced fluid accumulation in the control loops. However, these modulators did not enhance further C. jejuni‐induced fluid accumulation when injected along with C. jejuni live culture in the experimental loops. Both 1‐verapamil (100 μM) and PKC antagonist, H‐7 (15 μg/ml−1) significantly reduced C. jejuni‐induced fluid accumulation (P 0.05). Our findings suggest that both Ca2+ and PKC appear to be the important second messengers involved in the stimulation of intestinal fluid accumulation in C. jejuni infection.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1993.tb06406.x