Differential modulation of protein kinase C isoforms in erythroleukemia during induced differentiation

Induction of erythroid differentiation of murine erythroleukemia cells (MELC) by exposure to hexamethylene bisacetamide (HMBA) involves the modulation of protein kinase C (PKC) activity. Using immuno- and Northern blot techniques, we have demonstrated that MELC express a pattern of PKC isoforms whic...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1993-11, Vol.53 (22), p.5554-5558
Main Authors: LIN LENG, FEI YU, LIQUN DONG, BUSQUETS, X, SHIN-ICHI OSADA, RICHON, V. M, MARKS, P. A, RIFKIND, R. A
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Biological and medical sciences
Blotting, Northern
Cell Differentiation
Cell Line
General aspects
Immunoblotting
Isoenzymes - analysis
Isoenzymes - metabolism
Leukemia, Erythroblastic, Acute - enzymology
Leukemia, Erythroblastic, Acute - pathology
Medical sciences
Mice
Pharmacology. Drug treatments
Protein Kinase C - analysis
Protein Kinase C - metabolism
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Summary:Induction of erythroid differentiation of murine erythroleukemia cells (MELC) by exposure to hexamethylene bisacetamide (HMBA) involves the modulation of protein kinase C (PKC) activity. Using immuno- and Northern blot techniques, we have demonstrated that MELC express a pattern of PKC isoforms which includes PKC alpha, PKC delta, PKC epsilon, PKC zeta, and PKC eta. We show that MELC resistant to induction by HMBA express significantly less of the nPKC isoform, PKC delta, and slightly less PKC epsilon. Recovery of HMBA sensitivity is associated with reexpression of PKC delta protein. Upon exposure to HMBA, there is a fall in cytosolic PKC delta and PKC epsilon accompanied by a transient increase in membrane-associated forms of these PKC isoforms. HMBA-resistant MELC fail to display this isoform-specific translocation of PKC. Induction of differentiation is accompanied, over the next 24 h of exposure to HMBA, by a progressive fall in cellular PKC activity, associated with a progressive fall in the cellular content of PKC delta, PKC epsilon, and PKC zeta. These studies suggest that PKC delta, and possibly PKC epsilon and PKC zeta as well, play a role in the pathway of HMBA-mediated terminal cell differentiation of MELC.
ISSN:0008-5472
1538-7445