Analysis of mixed parasite populations of Theileria sergenti using cDNA probes encoding a major piroplasm surface protein

The gene for the 32 kDa surface protein (p32) of Theileria sergenti was cloned into λgt11 and its nucleotide sequence was determined. The gene encodes a protein of 283 amino acids as deduced from its nucleotide sequence with a 22 residue N-terminal signal peptide. Using this cDNA as a probe we have...

Full description

Saved in:
Bibliographic Details
Published in:Parasitology 1993-11, Vol.107 (4), p.369-377
Main Authors: Matsuba, T., Kubota, H., Tanaka, M., Hattori, M., Murata, M., Sugimoto, C., Onuma, M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid substitution
Animals
Base Sequence
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Blotting, Northern
Blotting, Southern
Cattle
Cattle Diseases - parasitology
Cloning, Molecular
DNA, Complementary
Fundamental and applied biological sciences. Psychology
Genes, Protozoan - genetics
Genetic Variation
Genome
Invertebrates
Membrane Proteins - genetics
mixed population
Molecular Sequence Data
Protozoa
Protozoan Proteins - genetics
Restriction Mapping
RNA, Protozoan - genetics
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Theileria - genetics
Theileria sergenti
Theileriasis - parasitology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The gene for the 32 kDa surface protein (p32) of Theileria sergenti was cloned into λgt11 and its nucleotide sequence was determined. The gene encodes a protein of 283 amino acids as deduced from its nucleotide sequence with a 22 residue N-terminal signal peptide. Using this cDNA as a probe we have isolated another two clones from a cDNA library with a CDM8 vector system derived from the same parasite stock. Comparison with three cDNA clones revealed differential polyadenylation and differences in sequences of non-coding regions. Within the coding regions, there were nucleotide transitions which affected the Pst I-restriction site, and one of the transitions was also accompanied by an amino acid substitution (Ala to Gly). Southern blot analysis showed hybridization pattern changes among the parasites isolated from individual calves at different times after infection. From these results, we conclude that at least 3 genetically different parasite populations may coexist, and that transition to predominant parasite populations might occur during persistent infections in a host, possibly to evade the host immune responses.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182000067718