Selective decontamination, induced colonization resistance and connected immunological changes in piglets

14-d-old conventional piglets were picked from normal piggery, washed with disinfectants, placed into isolators suitable for germfree work, fed a sterile diet and treated with peroral antibiotics (nalidixic acid, kanamycin, and nystatin). Beginning with day 5 or 7, Enterobacteriaceae were not found...

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Bibliographic Details
Published in:Folia microbiologica 1985-01, Vol.30 (3), p.312-318
Main Authors: MANDEL, L, TALAFANTOVA, M, TREBICHAVSKY, I, TRAVNICEK, J, KOUKAL, M
Format: Article
Language:English
Subjects:
Animals
Antibacterial agents
antibioticos
antibiotics
Antibiotics. Antiinfectious agents. Antiparasitic agents
antibiotique
bacteriose
bacterioses
bacteriosis
barrier husbandry
Biological and medical sciences
crianza protegida
Digestive System - microbiology
elevage protege
enterobacteriaceae
Germ-Free Life
Immunoglobulin A, Secretory - analysis
lechon
Leukocyte Count
Medical sciences
Pharmacology. Drug treatments
piglets
porcelet
Spleen - immunology
Swine - immunology
Swine - microbiology
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Summary:14-d-old conventional piglets were picked from normal piggery, washed with disinfectants, placed into isolators suitable for germfree work, fed a sterile diet and treated with peroral antibiotics (nalidixic acid, kanamycin, and nystatin). Beginning with day 5 or 7, Enterobacteriaceae were not found in feces. The absence of these bacteria was proved by inoculation of germfree newborn piglets with caecal content. In selectively decontaminated piglets, the white blood cell count in blood had fallen to 6 X 10(9)/L; this decrease was due to an extremely low number of granulocytes (to 0.8 X 10(9)/L). On day 35, IgG-positive cells almost disappeared from the spleen, whereas IgA cells were found in an unusually great amount. Corresponding changes in serum levels were established. The colonization resistance effect in Enterobacteriaceae-deprived piglets was confirmed; settling of introduced various E. coli strains did not occur or was delayed.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02923525