Characterization of autoantibodies to ferritin in canine serum

Ferritin-binding proteins circulating in mammalian blood are thought to be involved in the clearance of ferritin. The present study characterizes canine serum autoantibodies (IgM and IgA) that react with ferritin. Canine IgM and IgA bound to bovine spleen ferritin as well as canine liver ferritin. T...

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Bibliographic Details
Published in:Biometals 2002-06, Vol.15 (2), p.161-165
Main Authors: Orino, Koichi, Higa, Misaki, Fujikawa, Mika, Takehara, Kazuhiro, Okano, Shozo, Yamamoto, Shinji, Watanabe, Kiyotaka
Format: Article
Language:English
Subjects:
Animals
Antibody Specificity
Autoantibodies - blood
Autoantibodies - immunology
Baculovirus
Cattle
Dogs
Dogs - blood
Dogs - immunology
Ferritins - blood
Ferritins - chemistry
Ferritins - immunology
Immunoglobulin A - blood
Immunoglobulin A - immunology
Immunoglobulin M - blood
Immunoglobulin M - immunology
Immunoglobulins
Iron
Liver - chemistry
Myocardium - chemistry
Protein Subunits
Proteins
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Summary:Ferritin-binding proteins circulating in mammalian blood are thought to be involved in the clearance of ferritin. The present study characterizes canine serum autoantibodies (IgM and IgA) that react with ferritin. Canine IgM and IgA bound to bovine spleen ferritin as well as canine liver ferritin. To examine the specificity of canine IgM and IgA to ferritin H and L subunits, we used canine heart ferritin and canine liver ferritin with H/L subunit ratios of 3.69 and 0.43, respectively. Canine IgM and IgA recognized both of the H- and L-subunit-rich isoferritins, showing that their binding activities to ferritin depend on the H-subunit content. Recombinant bovine H-chain ferritin homopolymer expressed in a baculovirus expression system bound more with IgM and IgA than the recombinant L-chain homopolymer expressed under the same conditions. These results suggest that canine IgM and IgA recognize H-subunit-rich isoferritins, and that H-subunit-rich isoferritins are cleared from the circulation more rapidly than L-subunit-rich isoferritins.
ISSN:0966-0844
1572-8773
DOI:10.1023/A:1015239324414