Persistent Noncytopathic Infection of Normal Human T Lymphocytes with AIDS-Associated Retrovirus

Infection of normal peripheral blood T cells by the acquired immune deficiency syndrome (AIDS)--associated retrovirus (ARV) was evaluated in long-term cultures of helper-inducer T cells (T4 cells). Cells that were inoculated with ARV and maintained in medium supplemented with interleukin-2 remained...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1985-09, Vol.229 (4720), p.1400-1402
Main Authors: Hoxie, James A., Haggarty, Beth S., Rackowski, Jerome L., Pillsbury, Nan, Levy, Jay A.
Format: Article
Language:English
Subjects:
Acquired Immunodeficiency Syndrome - immunology
Acquired Immunodeficiency Syndrome - microbiology
AIDS
AIDS (Disease)
AIDS research
AIDS/HIV
Antigens
Antigens, Viral - immunology
Biological and medical sciences
Cells, Cultured
Cultured cells
Deltaretrovirus - immunology
Fundamental and applied biological sciences. Psychology
Giant cells
HTLV viruses
Humans
Infections
Lymphocytes
Microbiology
Microscopy, Fluorescence
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Retroviridae
Retroviridae Infections - immunology
Retroviridae Infections - microbiology
Retroviruses
T cells
T lymphocytes
T-Lymphocytes - microbiology
Viral antigens
Virology
Viruses
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Summary:Infection of normal peripheral blood T cells by the acquired immune deficiency syndrome (AIDS)--associated retrovirus (ARV) was evaluated in long-term cultures of helper-inducer T cells (T4 cells). Cells that were inoculated with ARV and maintained in medium supplemented with interleukin-2 remained productively infected with this virus for more than 4 months in culture, although they showed no cytopathic effects characteristic of acute ARV infection. The presence of replicating virus was demonstrated by reverse transcriptase activity of culture fluids and by viral antigens and budding particles detected on cells by immunofluorescence and electron microscopy. Virus produced in these cultures remained infectious and could induce cytopathic effects and viral antigens in uninfected lymphoid cells. The finding that normal lymphocytes may be productively infected by an AIDS retrovirus in the absence of cell death suggests that a range of biologic effects may occur after infection in vivo.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2994222