Genetics, subcellular localization, and molecular characterization of 6-phosphogluconate dehydrogenase isozymes in tomato

Three independent genes are responsible for 6-phosphogluconate dehydrogenase (6PGDH) activity in tomato. 6Pgdh-2, located on chromosome 12, codes for subunits of an active dimer which is restricted to the plastids. 6Pgdh-1, chromosome 4, and 6Pgdh-3, chromosome 5, code for subunits which form three...

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Bibliographic Details
Published in:Biochemical genetics 1985-06, Vol.23 (5/6), p.441-454
Main Authors: Tanksley, S.D, Kuehn, G.D
Format: Article
Language:English
Subjects:
6-PHOSPHOGLUCONATE DEHYDROGENASE
Agronomy. Soil science and plant productions
Alleles
Biological and medical sciences
Chloroplasts - enzymology
Chromosome Banding
Classical and quantitative genetics. Population genetics. Molecular genetics
Classical genetics, quantitative genetics, hybrids
Crosses, Genetic
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetics and breeding of economic plants
Genetics of eukaryotes. Biological and molecular evolution
ISOENZIMAS
ISOENZYME
ISOENZYMES
Isoenzymes - genetics
Isoenzymes - isolation & purification
Kinetics
LINKAGE
LYCOPERSICON ESCULENTUM
Macromolecular Substances
Molecular Weight
Phosphogluconate Dehydrogenase - genetics
Phosphogluconate Dehydrogenase - isolation & purification
Phosphogluconate Dehydrogenase - metabolism
Plants - enzymology
Pteridophyta, spermatophyta
Subcellular Fractions - enzymology
TOMATE
TOMATOES
Vegetals
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Summary:Three independent genes are responsible for 6-phosphogluconate dehydrogenase (6PGDH) activity in tomato. 6Pgdh-2, located on chromosome 12, codes for subunits of an active dimer which is restricted to the plastids. 6Pgdh-1, chromosome 4, and 6Pgdh-3, chromosome 5, code for subunits which form three dimers--two homodimers and an intergenic heterodimer. The latter three isozymes are found in the cytosol. 6Pgdh-1, 6Pgdh-2, and 6Pgdh-3 code for subunits with estimated molecular weights of 49,500, 50,500, and 51,200, respectively. The intergenic heterodimer encoded by 6Pgdh-1 and 6Pgdh-3 is thus composed of subunits that differ in length by approximately 15 amino acid residues. Divergence in the length and primary subunit structure may account for the lower thermal stability of the intergenic heterodimer compared with the corresponding homodimers. A limited survey of other solanaceous plant species suggests that the duplication of cytosolic 6PGDH-coding genes found in tomato may be widespread in the family.
ISSN:0006-2928
1573-4927
DOI:10.1007/BF00499085