Capsid destabilization is required for antibody-mediated disruption of poliovirus

• Published in: Journal of general virology 1994-03, Vol.75 (3), p.581-587
• Main Authors: Delaet, I, Boeye, A
• Format: Article
• Language: English
• Subjects: Antibodies, Monoclonal - immunology; Antibodies, Viral - immunology; Biological and medical sciences; Capsid - drug effects; Capsid - immunology; Capsid - metabolism; Fundamental and applied biological sciences. Psychology; Isoxazoles - pharmacology; Microbiology; Neutralization Tests; Osmolar Concentration; poliovirus; Poliovirus - drug effects; Poliovirus - immunology; Poliovirus - metabolism; Temperature; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-75-3-581

Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium Three out of 36 poliovirus type 1-specific monoclonal antibodies which, at 37 °C in a medium of normal ionic strength (µ = 0.16), caused only aggregative neutralization (reversible by immune complex dissociation at pH 2) shifted to cause disruptive, acid-irreversible neutralization when the temperature was raised to 39 °C or the ionic strength was lowered to 1/100 of normal. Under both conditions, the antigenic conversion was stoichiometric, but the efficiency was lower at 39 °C than at low ionic strength. Antigenic conversion and irreversible neutralization under both conditions were inhibited by WIN 51711, a capsid-stabilizing compound. Complete inhibition required filling of most of the virion's binding pockets by this compound. Received 13 August 1993; accepted 1 November 1993.