Effect of Tetracycline on the Attachment of K88+ Enterotoxigenic Escherichia coli to Porcine Small-Intestinal Cells

The attachment of six strains of K88+, porcine pathogenic, enterotoxigenic Escherichia coli to isolated porcine intestinal mucosal cells was decreased following growth in the presence of concentrations of oxytetracycline below the minimal inhibitory concentration (MIC). The decrease in binding by th...

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Bibliographic Details
Published in:The Journal of infectious diseases 1985-11, Vol.152 (5), p.1032-1036
Main Authors: Deneke, Carl F., Thorne, Grace M., Larson, A. D., Gorbach, Sherwood L.
Format: Article
Language:English
Subjects:
Action of physical and chemical agents on bacteria
Adhesiveness
Animals
Antibiotic resistance
Antibiotics
Antigens, Bacterial
Antigens, Surface
Bacterial adhesion
Bacteriology
Biological and medical sciences
Cell culture techniques
Cell lines
Cultured cells
Drug Resistance, Microbial
Enterotoxins - biosynthesis
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - immunology
Escherichia coli - physiology
Escherichia coli Proteins
Fimbriae Proteins
Fundamental and applied biological sciences. Psychology
Intestinal Mucosa - cytology
Intestinal Mucosa - microbiology
Intestine, Small - microbiology
Microbiology
Original Articles
Oxytetracycline - pharmacology
Pathogens
Plasmids
Swine
Therapeutic irrigation
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Summary:The attachment of six strains of K88+, porcine pathogenic, enterotoxigenic Escherichia coli to isolated porcine intestinal mucosal cells was decreased following growth in the presence of concentrations of oxytetracycline below the minimal inhibitory concentration (MIC). The decrease in binding by the wild-type strains was detected at concentrations of drug as low as 0.001 µg/ml, which was greater than four orders of magnitude below the MIC. When drug resistance was induced in these six strains, there was still a decrease in binding when the bacteria were grown in the presence of tetracycline. This decrease was comparable to the decrease in binding capacity of the wild-type strains caused by growth in the presence of tetracycline. In contrast, when one strain (G1108E) was made tetracycline resistant by the introduction of the R16 plasmid, the antibiotic had less effect on the binding of this strain than on the wild-type strain; however, growth in the presence of antibiotic still decreased adhesion. Overall, oxytetracycline decreased the adhesion of wild-type, induced-resistant, and genetically resistant K88+ enterotoxigenic E. coli to porcine small-intestinal cells, and this effect occurred at antibiotic concentrations several orders of magnitude below the MIC.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/152.5.1032