Oxy- and deoxyhaemoglobin adsorption onto glass and polymer surfaces

The adsorption of deoxyhaemoglobin (deoxyHb) and oxyhaemoglobin (oxyHb) was determined on clean glass, n-pentyl triethoxysilane (NPS)-treated glass, polystyrene (PS), and a polyetherurethane (PEU). The adsorbed amounts range from 0.1 to 0.6 μg/cm 2 for oxyHb and from 0.3 to 0.7 μg/cm 2 for deoxyHb....

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Bibliographic Details
Published in:Biomaterials 1985-07, Vol.6 (4), p.231-236
Main Authors: Chen, Jie, Andrade, J.D., VanWagenen, R.A.
Format: Article
Language:English
Subjects:
Adsorption
deoxyHb
Glass
glass surface
haemoglobin
Hemoglobins
Humans
Kinetics
Macromolecular Substances
oxyHb
Oxyhemoglobins
polymer surface
Polymers
Polystyrenes
Polyurethanes
Silanes
Surface Properties
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Summary:The adsorption of deoxyhaemoglobin (deoxyHb) and oxyhaemoglobin (oxyHb) was determined on clean glass, n-pentyl triethoxysilane (NPS)-treated glass, polystyrene (PS), and a polyetherurethane (PEU). The adsorbed amounts range from 0.1 to 0.6 μg/cm 2 for oxyHb and from 0.3 to 0.7 μg/cm 2 for deoxyHb. DeoxyHb adsorbs onto all these surfaces more than oxyHb. The more Hydrophobie the surface, the more adsorption of both deoxy and oxyHb forms. These results suggest the oxyHb and deoxyHb interact differently with the surfaces studied. It is likely that the surface hydrophobicity of Hb plays a major role in Hb adsorption onto surfaces; the deoxyHb surface is more Hydrophobie than the oxyHb surface. The binding sites for Hb adsorption may include the clefts between α 1, β 1,. A surface-induced dimerization mechanism is proposed to explain the adsorption of oxyHb.
ISSN:0142-9612
1878-5905
DOI:10.1016/0142-9612(85)90018-3