Xenopus laevis Ovarian DNA Helicase I: A 3' to 5' Helicase That Unwinds Short Duplexes

• Published in: Biochemistry (Easton) 1994-04, Vol.33 (13), p.3841-3847
• Main Authors: Poll, E. H. A, Harrison, Jeff, Umthun, Angela, Dobbs, Drena L, Benbow, Robert M
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphatases - metabolism; Analytical, structural and metabolic biochemistry; Animals; Base Sequence; Biological and medical sciences; Cations, Monovalent; DNA - chemistry; DNA - metabolism; DNA Helicases - metabolism; DNA-Binding Proteins - metabolism; Enzymes and enzyme inhibitors; Female; Fundamental and applied biological sciences. Psychology; Miscellaneous; Molecular Sequence Data; Oligodeoxyribonucleotides - chemistry; Ovary - enzymology; Structure-Activity Relationship; Substrate Specificity; Xenopus laevis
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00179a007

A novel DNA helicase isolated from Xenopus laevis ovaries [Poll, E. H. A., & Benbow, R. M. (1988) Biochemistry 27, 8701-8706] was characterized biochemically. The directionality of DNA unwinding was determined to be 3' to 5'. A short 3' ssDNA tail adjacent to duplex DNA was required for DNA unwinding; the minimum length of this tail was between four and nine bases. Only short duplex DNA regions were unwound: duplex DNA of 16 base pairs was readily unwound, whereas a 26 base pair duplex was not. Longer duplex regions were unwound in the presence of Escherichia coli single-strand DNA binding protein if, in addition, the duplex region was flanked by an unpaired 3' or 5' tail and the substrate resembled a branched replicative intermediate. X. laevis DNA helicase I exhibited high affinity for ssDNA, moderate affinity for dsDNA, and no affinity for RNA. DNA unwinding activity was stimulated by monovalent cations, with an optimal concentration of 150 mM for NaCl or KCl or 125 mM for Na chi PO4 or K chi PO4. The ATP analog ATP gamma S inhibited the DNA unwinding and copurifying DNA-dependent ATPase activity, whereas AMPPCP and AMPPNP moderately inhibited DNA unwinding activity and had little effect on the copurifying DNA-dependent ATPase activity. CTP was a relatively strong inhibitor of DNA unwinding activity, but GTP, UTP, dCTP, dGTP, or TTP showed moderate or no inhibition. The copurifying DNA-dependent ATPase activity was not inhibited by CTP, GTP, UTP, dCTP, dGTP, or TTP.