Involvement of calpain-I and microRNA34 in kanamycin-induced apoptosis of inner ear cells

Inner ear cells, including hair cells, spiral ganglion cells, stria vascularis cells and supporting cells on the basilar membrane, play a major role in transducing hearing signals and regulating inner ear homoeostasis. However, their functions are often damaged by antibiotic‐induced ototoxicity. Apo...

Full description

Saved in:
Bibliographic Details
Published in:Cell biology international 2010-12, Vol.34 (12), p.1219-1225
Main Authors: Yu, Li, Tang, Hao, Jiang, Xiao Hua, Tsang, Lai Ling, Chung, Yiu Wa, Chan, Hsiao Chang
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - adverse effects
Anti-Bacterial Agents - pharmacology
apoptosis
Apoptosis - drug effects
Apoptosis - genetics
calpain
Calpain - genetics
Calpain - metabolism
Calpain - physiology
Cells - drug effects
Cells - metabolism
Ear, Inner - drug effects
Ear, Inner - metabolism
Ear, Inner - pathology
Gene Expression - drug effects
In Situ Nick-End Labeling
kanamycin
Kanamycin - adverse effects
Kanamycin - pharmacology
Male
Mice
Mice, Inbred BALB C
microRNA
MicroRNAs - genetics
MicroRNAs - metabolism
MicroRNAs - physiology
ototoxicity
Time Factors
Up-Regulation - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Inner ear cells, including hair cells, spiral ganglion cells, stria vascularis cells and supporting cells on the basilar membrane, play a major role in transducing hearing signals and regulating inner ear homoeostasis. However, their functions are often damaged by antibiotic‐induced ototoxicity. Apoptosis is probably involved in inner ear cell injury following aminoglycoside treatment. Calpain, a calcium‐dependent protease, is essential for mediating and promoting cell death. We have therefore investigated the involvement of calpain in the molecular mechanism underlying ototoxicity induced by the antibiotic kanamycin in mice. Kanamycin (750 mg/kg) mainly induced cell death of cochlear cells, including stria vascularis cells, supporting cells and spiral ganglion cells, but not hair cells within the organ of Corti. Cell death due to apoptosis occurred in a time‐dependent manner with concomitant up‐regulation of calpain expression. Furthermore, the expression levels of two microRNAs, mir34a and mir34c, were altered in a dose‐dependent manner in cochlear cells. These novel findings demonstrated the involvement of both calpain and microRNAs in antibiotic‐induced ototoxicity.
ISSN:1065-6995
1095-8355
DOI:10.1042/CBI20100515