Tubulin evolution: ciliate-specific epitopes are conserved in the ciliary tubulin of metazoa

In spite of their overall evolutionary conservation, the tubulins of ciliates display electrophoretic and structural particularities. We show here that antibodies raised against Paramecium and Tetrahymena ciliary tubulins fail to recognize the cytoplasmic tubulins of all the metazoans tested. Immuno...

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Bibliographic Details
Published in:Journal of molecular evolution 1985-01, Vol.22 (3), p.220-229
Main Authors: ADOUTTE, A, CLAISSE, M, MAUNOURY, R, BEISSON, J
Format: Article
Language:English
Subjects:
Animals
Antigen-Antibody Complex
Biological and medical sciences
Biological Evolution
Chickens
Cilia - analysis
Epitopes - analysis
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Humans
Mice
Paramecium
Paramecium - genetics
Peptide Fragments - analysis
Physarum - genetics
Species Specificity
Swine
Tetrahymena
Tetrahymena - genetics
Tubulin - genetics
Tubulin - immunology
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Summary:In spite of their overall evolutionary conservation, the tubulins of ciliates display electrophoretic and structural particularities. We show here that antibodies raised against Paramecium and Tetrahymena ciliary tubulins fail to recognize the cytoplasmic tubulins of all the metazoans tested. Immunoblotting of peptide maps of ciliate tubulins reveals that these antibodies react with one or very few ciliate-specific epitopes, in contrast to polyclonal antibodies against vertebrate tubulins, which are equivalent to autoantibodies and recognize several epitopes in both ciliate and vertebrate tubulins. Furthermore, we show that the anti-ciliate antibodies recognize ciliary and flagellar tubulins of metazoans ranging from sea urchin to mammals (with the exception of humans). The results support the conclusion that although duplication and specialization of tubulin genes in metazoans may have led to distinct types of tubulins, the axonemal one has remained highly conserved.
ISSN:0022-2844
1432-1432
DOI:10.1007/BF02099751