Candidacidal Activity of Macrophages from Immunocompetent and Congenitally Immunodeficient Mice

The chemotactic, phagocytic, and candidacidal activities of peritoneal exudate macrophages from immunocompetent heterozygous (bg/+) and immunodeficient homozygous (bg/bg, bg/bg-nu/+, and bg/bg-nu/nu) beige mice were assessed. Overall, macrophages from all strains of mice tested not only were able to...

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Bibliographic Details
Published in:The Journal of infectious diseases 1994-07, Vol.170 (1), p.180-188
Main Authors: Vazquez-Torres, Andres, Jones-Carson, Jessica, Balish, Edward
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Bacteriophages
Candida albicans
Candida albicans - immunology
Candidiasis
Cells, Cultured
Chemotaxis
Concanavalin A - pharmacology
Hot Temperature
Immunocompetence - immunology
Immunologic Deficiency Syndromes - congenital
Immunologic Deficiency Syndromes - immunology
Infections
Interferon-gamma - pharmacology
Lipopolysaccharides - pharmacology
Macrophages
Macrophages, Peritoneal - cytology
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - immunology
Major Articles
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
Mice, Nude
Mutation
Neutrophils
Peritoneal macrophages
Phagocytes
Phagocytosis
Splenocytes
Superoxide Dismutase - metabolism
Thioglycolates - pharmacology
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Summary:The chemotactic, phagocytic, and candidacidal activities of peritoneal exudate macrophages from immunocompetent heterozygous (bg/+) and immunodeficient homozygous (bg/bg, bg/bg-nu/+, and bg/bg-nu/nu) beige mice were assessed. Overall, macrophages from all strains of mice tested not only were able to migrate into the peritoneal cavity in response to several eliciting agents but showed a comparable capacity to phagocytize fluorescein isothiocyanate-labeled, heat-killed Candida albicans. However, some populations of peritoneal exudate macrophages from homozygous beige mice (e.g., thioglycollate-elicited) and resident peritoneal macrophages from bg/bg mice incubated in vitro with supernatants from concanavalin A-stimulated splenocytes had poorer candidacidal activity than did control macrophages from bg/+ mice. Interferon-γ enhanced the in vitro candidacidal activity of macrophages from homozygous and heterozygous beige mice. As indicated by inhibitors, poor macrophage candidacidal activity seemed to correlate better with deficient nitric oxide- than with superoxide anion-mediated killing. These data suggest that impaired candidacidal activity of macrophages from homozygous beige mice may explain their enhanced susceptibility to candidiasis.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/170.1.180