Major expansion of CD8 + T cells with a predominant Vβ usage during the primary immune response to HIV

A SIGNIFICANT proportion (up to 70%) of individuals experience an acute clinical syndrome of varying severity associated with primary infection with the human immunodeficiency virus (HIV) 1–4 . We report here studies on six individuals who showed an acute HIV syndrome which generally resolved within...

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Bibliographic Details
Published in:Nature (London) 1994-08, Vol.370 (6489), p.463-467
Main Authors: Pantaleo, Giuseppe, Demarest, James F, Soudeyns, Hugo, Graziosi, Cecilia, Denis, François, Adelsberger, Joseph W, Borrow, Persephone, Saag, Michael S, Shaw, George M, Sekalytt, Rafick P, Fauci, Anthony S
Format: Article
Language:English
Subjects:
Adolescent
Adult
AIDS/HIV
Amino Acid Sequence
Biological and medical sciences
CD8 Antigens
Cell Line, Transformed
Cells, Cultured
Clone Cells
Cytotoxicity, Immunologic
DNA
Female
Flow Cytometry
HIV
HIV - immunology
HIV Infections - immunology
Human immunodeficiency virus
Humanities and Social Sciences
Humans
Immune response
Immunity (Disease)
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunogenetics
Immunopathology
Immunophenotyping
letter
Lymphocytes
Male
Medical research
Medical sciences
Molecular Sequence Data
multidisciplinary
Polymerase Chain Reaction
Prospective Studies
Receptors, Antigen, T-Cell, alpha-beta - genetics
Science
Science (multidisciplinary)
T-Lymphocyte Subsets - immunology
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Summary:A SIGNIFICANT proportion (up to 70%) of individuals experience an acute clinical syndrome of varying severity associated with primary infection with the human immunodeficiency virus (HIV) 1–4 . We report here studies on six individuals who showed an acute HIV syndrome which generally resolved within four weeks, concomitant with a dramatic downregulation of viraemia 2–5 . To characterize the T-cell-mediated primary immune response to HIV, we used combined semiquantitative polymerase chain reaction assay and cytofluorometry to analyse the T-cell antigen receptor repertoire in sequential peripheral blood mononuclear cells from the patients. We found major oligoclonal expansions in a restricted set of variable-domain β-chain (Vβ) families. Cells expressing the expanded Vβs predominantly expressed the CDS T-cell differentiation antigen and mediated HlV-specific cytotoxicity. Major oligoclonal expansions of these CD8 + T lymphocytes may represent an important component of the primary immune response to viral infections and may help to clarify both the immunopathogenic and the protective mechanisms of HIV infection.
ISSN:0028-0836
1476-4687
DOI:10.1038/370463a0