Production of Slime Polysaccharides by Shigella dysenteriae Type 1

Electron microscopy of ruthenium red-stained ultrathin section of strains of Shigella dysenteriae type 1 grown in the Casamino Acids-yeast extract broth medium showed the presence of an extracellular slime layer. The slime appeared as a dense sheath covering bacteria. The presence of slime promoted...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1994, Vol.38(1), pp.11-18
Main Authors: Qadri, Firdausi, Haque, Md. Azizul, Hossain, Anwar, Albert, Manuel John
Format: Article
Language:English
Subjects:
Animals
Bacterial Adhesion
Bacteriology
Biological and medical sciences
Enzyme-Linked Immunosorbent Assay
Extracellular Space - metabolism
Fundamental and applied biological sciences. Psychology
Glycoproteins - chemistry
Glycoproteins - physiology
Guinea Pigs - blood
Hemagglutination
Hemagglutination Tests
Immunodiffusion
Lipopolysaccharides - chemistry
Lipopolysaccharides - isolation & purification
Microbiology
Microscopy, Electron
Molecular Weight
Morphology, structure, chemical composition
Polysaccharides - chemistry
Polysaccharides - physiology
Polysaccharides, Bacterial - biosynthesis
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - immunology
Ruthenium Red
Shigella dysenteriae
Shigella dysenteriae - immunology
Shigella dysenteriae - metabolism
Shigella dysenteriae - ultrastructure
Slime polysaccharide
Staining and Labeling
Sugar Acids - analysis
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Summary:Electron microscopy of ruthenium red-stained ultrathin section of strains of Shigella dysenteriae type 1 grown in the Casamino Acids-yeast extract broth medium showed the presence of an extracellular slime layer. The slime appeared as a dense sheath covering bacteria. The presence of slime promoted hemagglutinating activity of the bacteria. The slime polysaccharide (SPS) isolated from the cell-free culture supernatant or the bacterial surface was less than 162, 000 daltons in size and immunochemically similar. The SPS showed cross-reaction with lipopolysaccharide (LPS) antigen in immunological tests; however, it also appeared to be different from LPS since it did not contain 2-keto-3-deoxyoctonate, a core sugar of LPS. A different pattern of separation from LPS was also observed by silver staining of SDS-polyacrylamide gels. From these data it appeared that either LPS and SPS are contaminated with each other or that SPS is the polysaccharide portion of LPS.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1994.tb01738.x