Structural Order of Membranes and Composition of Phospholipids in Fish Brain Cells During Thermal Acclimatization

• Published in: Proceedings of the National Academy of Sciences - PNAS 1994-08, Vol.91 (17), p.8234-8238
• Main Authors: Buda, Csaba, Dey, Indranil, Balogh, Nandor, Horvath, Laszlo I., Maderspach, Katalin, Juhasz, Miklos, Yeo, Young K., Farkas, Tibor
• Format: Article
• Language: English
• Subjects: Acclimatization; Acclimatization - physiology; Animals; Anisotropy; Biological Evolution; Brain; Brain - physiology; Carp; Carps - physiology; Cell Membrane - physiology; Cell Membrane - ultrastructure; Cell membranes; Chemical composition; Cyprinus carpio; Fatty acids; Fatty Acids - analysis; Fish; Fishes - physiology; Fluorescence Polarization; Freshwater; Lipids; Membrane Lipids - chemistry; Membrane Lipids - isolation & purification; Membrane Lipids - metabolism; Membranes; Neurons; Phosphatidylethanolamines; Phospholipids; Phospholipids - chemistry; Phospholipids - isolation & purification; Phospholipids - metabolism; Species Specificity; Temperature
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.91.17.8234

A comparison of the structural orders of membranes of a mixed brain-cell population isolated from Cyprinus carpio L. acclimated to either summer (23-25⚬C) or winter (5⚬C) revealed a high degree of compensation (80%) for temperature, as assayed by electron spin resonance spectroscopy. The cells rapidly forget their thermal history and adjust the physical properties of the membranes when shifted to the other extreme of temperature either in vivo or in vitro. Phospholipids separated from both types of animals exhibit only around 10% compensation. Arachidonic and docosahexaenoic acids are the major polyunsaturated fatty acids in the brains, but the fatty acid composition of the brain total phospholipids does not vary with adaptation to temperature. Separation of phosphatidylcholines and phosphatidylethanolamines into molecular species revealed a 2- to 3-fold accumulation of 18:1/22:6, 18:1/20:4, and 18:1/18:1 species in the latter; 18:0/22:6 showed an opposite tendency. Molecular species composition of phosphatidylcholines did not vary with the temperature. The same trends of changes were seen with brains of freshwater fish from subtropical (Catla catla L.) or boreal (Acerina cernua) regions. It is concluded that the gross amount of docosahexaenoic acid (22:6) plays only a minor role in adjusting the membrane physical properties to temperature. Factors other than lipids might be involved in the adaptation processes. Due to their specific molecular architecture, molecules such as 18:1/22:6, 18:1/20:4, or 18:1/18:1 phosphatidylethanolamine might prevent the contraction of membranes in the cold and may provide an environment for some other components involved in the temperature regulation of physical properties of nerve cell membranes.