Immunolocalization of complement C1s and matrix metalloproteinase 9 (92kDa gelatinase/type IV collagenase) in the primary ossification center of the human femur

The first component of complement C1s has been shown to degrade type I and type II collagens (Yamaguchi et al. 1990), the latter of which is a major constituent of the cartilage matrix. In order to understand the physiological roles of C1s in cartilage resorption, the expression of C1s was examined...

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Bibliographic Details
Published in:Cell and tissue research 1994-08, Vol.277 (2), p.239-245
Main Authors: SAKIYAMA, H, INABA, N, TOYOGUCHI, T, OKADA, Y, MATSUMOTO, M, MORIYA, H, OHTSU, H
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Biological and medical sciences
Bone Marrow - embryology
Bone Marrow - enzymology
Bone Marrow - metabolism
Bone Matrix - embryology
Bone Matrix - enzymology
Bone Matrix - metabolism
Cartilage - embryology
Cartilage - enzymology
Cartilage - metabolism
Collagenases - immunology
Collagenases - metabolism
Complement C1s - immunology
Complement C1s - metabolism
Enzyme Activation
Femur - embryology
Femur - enzymology
Femur - metabolism
Fundamental and applied biological sciences. Psychology
Humans
Immunohistochemistry
Matrix Metalloproteinase 9
Skeleton and joints
Space life sciences
Vertebrates: osteoarticular system, musculoskeletal system
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Summary:The first component of complement C1s has been shown to degrade type I and type II collagens (Yamaguchi et al. 1990), the latter of which is a major constituent of the cartilage matrix. In order to understand the physiological roles of C1s in cartilage resorption, the expression of C1s was examined by immunohistochemistry in the primary ossification center where the matrix is removed and replaced by bone marrow. Hypertrophic chondrocytes, endothelium and hematogenous elements in the capillary buds were intensely stained by a monoclonal antibody against C1s. Matrix metalloproteinase 9 (MMP-9, 92kDa gelatinase/type IV collagenase) was also immunolocalized in hypertrophic chondrocytes, mesenchymal cells in the primitive bone marrow and the cartilage matrix adjacent to the marrow. In addition, C1s was found to activate the zymogen of MMP-9. These observations suggest that C1s and MMP-9 coordinately participate in matrix degradation in cartilage.
ISSN:0302-766X
1432-0878
DOI:10.1007/s004410050151