Cellular Retinoic Acid-Binding Protein and Its Relationship to the Biological Activity of Four Synthetic Retinoids in Hamster Tracheal Organ Culture

The mechanism of action of retinoid in reversing keratinization in hamster trachea is yet unknown. The purpose of this study was to determine if cellular retinoic acid binding protein (CRABP) is present in tracheal epithelium following incubation in serum-free, vitamin A-deficient culture medium for...

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Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology 1986-03, Vol.22 (3), p.164-168
Main Authors: Mehta, Rajendra G., Schiff, Leonard J., Moore, Steven J., Ann Marie Buckley, Dawson, Marcia I.
Format: Article
Language:English
Subjects:
Animal cells
Animals
Binding sites
Binding, Competitive
Biological and medical sciences
Biotechnology
Carrier proteins
Carrier Proteins - metabolism
Cell Differentiation - drug effects
Cricetinae
Cytosol
Cytosol - metabolism
Diet
Epithelium
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Hamsters
Keratinization
Keratins - metabolism
Methods. Procedures. Technologies
Miscellaneous
Organ Culture Techniques
Rapid Communications in Cell Biology
Receptors, Retinoic Acid
Retinoids
Retinoids - metabolism
Retinoids - pharmacology
Trachea
Trachea - cytology
Trachea - drug effects
Trachea - metabolism
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Summary:The mechanism of action of retinoid in reversing keratinization in hamster trachea is yet unknown. The purpose of this study was to determine if cellular retinoic acid binding protein (CRABP) is present in tracheal epithelium following incubation in serum-free, vitamin A-deficient culture medium for 10 days, and if the effectiveness of a retinoid in reversing keratinization in organ culture is correlated with its ability to compete for CRABP sites. The cytosol prepared from tracheal cultures contained CRABP at a concentration of 2.61 pmoles per mg protein. Of the four retinoids with carboxyl end group selected for the study, two of the biological active retinoids competed for the CRABP sites. However, no correlation was observed between the biological activity of the inactive retinoids and their ability to associate with the CRABP sites. These results indicate that even though the action of retinoid may be mediated by retinoid binding protein, it cannot be used as a sole predicator of retinoid response in hamster trachea.
ISSN:0883-8364
2327-431X
1475-2689
DOI:10.1007/bf02623504