The closely related citrus ringspot and citrus psorosis viruses have particles of novel filamentous morphology

1 Universidad Nacional de La Plata, Instituto de Bioquimica y Biologia Molecular, 2 Universidad Nacional de La Plata, Catedra de Fitopatologia, 1900 La Plata, Argentina and 3 Istituto di Fitovirologia Applicata, CNR, Strada delle Cacce 73, I-10135 Torino, Italy Some properties of the particles of ci...

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Bibliographic Details
Published in:Journal of general virology 1994-12, Vol.75 (12), p.3585-3590
Main Authors: Garcia, Maria Laura, Dal Bo, Elena, Grau, Oscar, Milne, Robert G
Format: Article
Language:English
Subjects:
Antibodies, Viral
Capsid - analysis
Citrus - virology
Microscopy, Electron
Microscopy, Immunoelectron
Plant Viruses - classification
Plant Viruses - immunology
Plant Viruses - isolation & purification
Plant Viruses - ultrastructure
Ribulose-Bisphosphate Carboxylase
Virion - ultrastructure
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Summary:1 Universidad Nacional de La Plata, Instituto de Bioquimica y Biologia Molecular, 2 Universidad Nacional de La Plata, Catedra de Fitopatologia, 1900 La Plata, Argentina and 3 Istituto di Fitovirologia Applicata, CNR, Strada delle Cacce 73, I-10135 Torino, Italy Some properties of the particles of citrus ringspot virus (CtRSV) and the related citrus psorosis-associated virus (CPsAV) are described. The particles of CtRSV have been reported to be sinuous linear structures about 10 nm in diameter and of two lengths (300 to 500 nm and 1500 to 2500 nm) representing ‘top’ and ‘bottom’ sedimentation components. We show that these particles are collapsed double-stranded forms of nucleocapsid-like, highly flexuous open circles formed of filaments 3 to 4 nm in diameter. Top-component filaments had contour lengths of 600 to 1000 nm, i.e. twice that reported for the corresponding collapsed form. Bottom-component filaments had contour lengths about four times longer than those of top-component filaments. The structures suggest that CtRSV represents a new genus (possibly family) related to the tenuiviruses. However, we failed to demonstrate any serological relationship between CtRSV and several tenuiviruses; moreover, the capsid protein sizes and host ranges are quite different. We offer the name Ophiovirus for the proposed new genus. Received 5 April 1994; accepted 19 August 1994.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-75-12-3585