Differences Related to the Production of Arachidonic Acid between Collagen- and Thrombin-Stimulated Human Platelets

The effects of collagen and thrombin on the liberation of free arachidonic acid were investigated in human platelets by fluorometric high-performance liquid chromatography. Collagen induced a concentration-dependent increase in the extent of platelet aggregation, as well as an accumulation of arachi...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1994-08, Vol.116 (2), p.269-273
Main Authors: Fujita, Masamichi, Nakagawa, Yasuhito, Osawa, Ryoichi, Kijima-Suda, Isao, Setaka, Morio, Nojima, Shoshichi, Tomita, Kenkichi
Format: Article
Language:English
Subjects:
A23187
arachidonic acid
Arachidonic Acid - biosynthesis
Arachidonic Acid - blood
Arachidonic Acid - physiology
Blood Platelets - drug effects
Blood Platelets - metabolism
Calcimycin - pharmacology
Chromatography, High Pressure Liquid - methods
collagen
Collagen - pharmacology
ETYA
Fatty Acids - blood
Humans
Kinetics
Platelet Aggregation - drug effects
Platelet Aggregation - physiology
Stimulation, Chemical
thrombin
Thrombin - pharmacology
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Summary:The effects of collagen and thrombin on the liberation of free arachidonic acid were investigated in human platelets by fluorometric high-performance liquid chromatography. Collagen induced a concentration-dependent increase in the extent of platelet aggregation, as well as an accumulation of arachidonic acid in human platelets. By contrast, thrombin effectively provoked a potent aggregation at relatively low concentration without any accumulation of free arachidonic acid, although the accumulation of arachidonic acid was detected at a high concentration of thrombin (> 0.1 U/ml) that induced full aggregation. The selective liberation of arachidonic acid was found in thrombin-stimulated platelets. Non-selective liberation of fatty acids occurred in platelets that had been stimulated with a high concentration of collagen (10 μg/ml), as well as in platelets stimulated with A23187. The net amount of free arachidonic acid in collagen-stimulated platelets was estimated by use of eicosatetraenoic acid (ETYA), which is an inhibitor of both cyclooxygenase and lipoxygenase. ETYA markedly potentiated the accumulation of free arachidonic acid in collagen-stimulated platelets without changing the amounts of other fatty acids in the cell.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a124518