Genetics and biochemistry of dehalogenating enzymes

Microorganisms that can utilize halogenated compounds as a growth substrate generally produce-enzymes whose function is carbon-halogen bond cleavage. Based on substrate range, reaction type and gene sequences, the dehalogenating enzymes can be classified in different groups, including hydrolytic deh...

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Bibliographic Details
Published in:Annual review of microbiology 1994, Vol.48 (1), p.163-191
Main Authors: JANSSEN, D. B, PRIES, F, VAN DER PLOEG, J. R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteria - enzymology
Bacteria - genetics
Bacteriology
Biochemistry
Biological and medical sciences
Biological Evolution
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Carbon
Chlorine
Dechlorination
Enzymes
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetics
Glutathione Transferase - metabolism
Halogenated compounds
Halogens
Hydrocarbons, Halogenated - metabolism
Hydrolases - genetics
Hydrolases - metabolism
Lyases - genetics
Lyases - metabolism
Metabolism. Enzymes
Microbial enzymes
Microbial metabolism
Microbiology
Microorganisms
Mission oriented research
Molecular Sequence Data
Oxygenases - metabolism
Physiological aspects
Physiology and metabolism
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Summary:Microorganisms that can utilize halogenated compounds as a growth substrate generally produce-enzymes whose function is carbon-halogen bond cleavage. Based on substrate range, reaction type and gene sequences, the dehalogenating enzymes can be classified in different groups, including hydrolytic dehalogenases, glutathione transferases, monooxygenases and hydratases. X-ray crystallographic and biochemical studies have provided detailed mechanistic insight into the action of haloalkane dehalogenase. The essential features are nucleophilic substitution of the halogen by a carboxylate group and the presence of a distinct halogen binding site, formed by tryptophan residues. This review summaries current knowledge on a variety of other dehalogenating enzymes and indicates the existence of a widespread and diverse microbial potential for dechlorination of natural and xenobiotic halogenated compounds.
ISSN:0066-4227
1545-3251
DOI:10.1146/annurev.mi.48.100194.001115