Native chicken gizzard tropomyosin is predominantly a beta gamma-heterodimer

Unmodified chicken gizzard tropomyosin (TM) has been fractionated into its two major isoforms beta and gamma, by chromatofocussing in the presence of 9 M urea and dithiothrieitol. Treatment of the native protein with several bifunctional N-hydroxysuccinimide esters gave the beta gamma-heterodimer as...

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Bibliographic Details
Published in:The Journal of biological chemistry 1986-09, Vol.261 (27), p.12774-12778
Main Authors: Sanders, C, Burtnick, L D, Smillie, L B
Format: Article
Language:English
Subjects:
Animals
Chickens
Chromatography, High Pressure Liquid
Dithiothreitol - pharmacology
Electrophoresis, Polyacrylamide Gel
gesier
gizzard
Gizzard, Avian - enzymology
globulinas
globuline
globulins
Isoelectric Focusing
Macromolecular Substances
molleja estomago
muscle
muscles
musculos
pollo
poulet
Temperature
Tropomyosin - analysis
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Summary:Unmodified chicken gizzard tropomyosin (TM) has been fractionated into its two major isoforms beta and gamma, by chromatofocussing in the presence of 9 M urea and dithiothrieitol. Treatment of the native protein with several bifunctional N-hydroxysuccinimide esters gave the beta gamma-heterodimer as the major cross-linked product. A comparison of the thermal transition profiles of the two homodimers and of the native unfractionated TM also indicated the predominance of the beta gamma-heterodimer in the native protein. This conclusion is consistent with the absence of excimer fluorescence in pyrene-labeled gizzard TM and the relative resistance of the molecule to intramolecular disulfide formation (Lehrer, S.S., Betteridge, D.R., Graceffa, P., Wong, S., and Seidel, J. C. (1984) Biochemistry 23, 1591-1595) since the single cysteines on each of the two isoforms are widely separated. We conclude that further experimental evidence is required to assess the possibility that the gizzard TM is more rigid in its conformation than are those of the skeletal and cardiac proteins.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)67160-1