Translation control: bridging the gap between genomics and proteomics?

mRNA profiling enables the expression levels of thousands of transcripts in a cell to be monitored simultaneously. Nevertheless, analyses in yeast and mammalian cells have demonstrated that mRNA levels alone are unreliable indicators of the corresponding protein abundances. This discrepancy between...

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Bibliographic Details
Published in:Trends in biochemical sciences (Amsterdam. Regular ed.) 2001-04, Vol.26 (4), p.225-229
Main Authors: Pradet-Balade, Bérengère, Boulmé, Florence, Beug, Hartmut, Müllner, Ernst W, Garcia-Sanz, Jose A
Format: Article
Language:English
Subjects:
functional genomics
Gene Expression Profiling
Genome
mRNA expression profiling
polysome-bound mRNA
Protein Biosynthesis
Proteome
proteomics
RNA, Messenger - genetics
translationsl control
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Summary:mRNA profiling enables the expression levels of thousands of transcripts in a cell to be monitored simultaneously. Nevertheless, analyses in yeast and mammalian cells have demonstrated that mRNA levels alone are unreliable indicators of the corresponding protein abundances. This discrepancy between mRNA and protein levels argues for the relevance of additional control mechanisms besides transcription. As translational control is a major mechanism regulating gene expression, the use of translated mRNA in profiling experiments might depict the proteome more closely than does the use of total mRNA. This would combine the technical potential of genomics with the physiological relevance of proteomics.
ISSN:0968-0004
1362-4326
DOI:10.1016/S0968-0004(00)01776-X