A "CAT" family of repetitive DNA sequences in Saccharomyces cerevisiae

An oligonucleotide probe was used to isolate yeast genomic clones containing DNA sequences with repetitive elements consisting primarily of a tandemly arranged trinucleotide, CAT. Hybridization analyses estimate that the yeast genome contains 40-50 CAT clusters, representing the first repetitive DNA...

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Bibliographic Details
Published in:The Journal of biological chemistry 1986-10, Vol.261 (29), p.13401-13403
Main Authors: Wildeman, A G, Rasquinha, I, Nazar, R N
Format: Article
Language:English
Subjects:
Base Composition
Biological and medical sciences
DNA, Fungal - genetics
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Fungal
Genes. Genome
GENOMAS
GENOME
GENOMES
HIBRIDACION
HYBRIDATION
HYBRIDIZING
Molecular and cellular biology
Molecular genetics
Nucleic Acid Hybridization
NUCLEOTIDE
NUCLEOTIDES
NUCLEOTIDOS
Repetitive Sequences, Nucleic Acid
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Species Specificity
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Summary:An oligonucleotide probe was used to isolate yeast genomic clones containing DNA sequences with repetitive elements consisting primarily of a tandemly arranged trinucleotide, CAT. Hybridization analyses estimate that the yeast genome contains 40-50 CAT clusters, representing the first repetitive DNA sequence family found in yeast. Sequence analyses show short spacers between the CAT repeats consisting of closely related trinucleotides, primarily CGT. Some of the CAT clusters are located in longer repeating elements with lengths of 7 nucleotides or more. In one case a three-times-repeated 27-nucleotide sequence bears striking homology to the 21-base pair repeat region of the mammalian simian virus 40 promoter element. Hybridization studies further suggest that the "CAT" sequences may be widely dispersed in many diverse organisms including Escherichia coli, Drosophila, and man.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)67030-9