CXCR3 Chemokine Receptor Distribution in Normal and Inflamed Tissues: Expression on Activated Lymphocytes, Endothelial Cells, and Dendritic Cells

• Published in: Laboratory investigation 2001-03, Vol.81 (3), p.409-418
• Main Authors: García-López, María Ángeles, Sánchez-Madrid, Francisco, Rodríguez-Frade, Jose Miguel, Mellado, Mario, Acevedo, Agustín, García, M Isabel, Albar, Juan Pablo, Martínez-A, Carlos, Marazuela, Mónica
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal; Antibody Specificity; Biological and medical sciences; Cell physiology; Cells, Cultured; Chemotaxis, Leukocyte - immunology; Dendritic Cells - chemistry; Dendritic Cells - immunology; Endothelium, Vascular - chemistry; Endothelium, Vascular - immunology; Fundamental and applied biological sciences. Psychology; Humans; Inflammation; Kidney - cytology; Laboratory Medicine; Lymphocyte Activation - immunology; Lymphocytes - chemistry; Lymphocytes - immunology; Lymphoid Tissue - chemistry; Lymphoid Tissue - immunology; Lymphoid Tissue - pathology; Medicine; Medicine & Public Health; Mice; Molecular and cellular biology; Motility and taxis; Pathology; Receptors, Chemokine - analysis; Receptors, Chemokine - genetics; Receptors, Chemokine - immunology; Receptors, CXCR3; Synovitis - immunology; Synovitis - pathology; Thyroiditis, Autoimmune - immunology; Thyroiditis, Autoimmune - pathology; Transfection; Vasculitis - immunology; Vasculitis - pathology
• ISSN: 0023-6837; 1530-0307
• DOI: 10.1038/labinvest.3780248

Using new human CXCR3 chemokine receptor–specific monoclonal antibodies, we studied human CXCR3 tissue distribution in lymphoid and nonlymphoid organs, as well as in inflammatory conditions, including rheumatoid arthritis, Hashimoto's thyroiditis, and dermal vasculitis. CXCR3 was expressed by certain dendritic cell subsets, specifically myeloid-derived CD11c positive cells, not only in those present in normal lymphoid organs, but also in germinal centers generated in inflammatory conditions. CXCR3 expression was also detected in some lymphocyte subsets such as intraepithelial lymphocytes of secondary lymphoid organs and infiltrating lymphocytes in inflammatory conditions. In addition, CXCR3 was constitutively expressed by endothelial cells (EC) of vessels of medium and large caliber but not in small vessels from different organs. Finally, enhanced CXCR3 expression was found in EC and in infiltrating lymphocytes with an activated phenotype in inflammatory diseases. The CXCR3 chemokine receptor may play a role in the regulation of leukocyte migration to inflammatory sites.