Cloning and sequence analysis of cDNA for bovine carboxypeptidase E

Carboxypeptidase E (enkephalin convertase) was first identified as the carboxypeptidase B-like enzyme involved in the biosynthesis of enkephalin in bovine adrenal chromaffin granules 1 . A similar enzyme is present in many brain regions 1,2 and in purified secretory granules from rat pituitary 3 and...

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Bibliographic Details
Published in:Nature (London) 1986-10, Vol.323 (6087), p.461-464
Main Authors: Fricker, Lloyd D., Evans, Chris J., Esch, Fred S., Herbert, Edward
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Biotechnology
carboxypeptidase E
Carboxypeptidase H
Carboxypeptidases - genetics
Cattle
cDNA
Cloning, Molecular
DNA
DNA Restriction Enzymes
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Genetic engineering
Genetic technics
Humanities and Social Sciences
letter
Methods. Procedures. Technologies
Molecular and cellular biology
Molecular genetics
multidisciplinary
Nucleic Acid Hybridization
nucleotide sequence
Pituitary Gland - metabolism
restriction endonuclease mapping
Science
Science (multidisciplinary)
Sequence Homology, Nucleic Acid
Synthetic digonucleotides and genes. Sequencing
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Summary:Carboxypeptidase E (enkephalin convertase) was first identified as the carboxypeptidase B-like enzyme involved in the biosynthesis of enkephalin in bovine adrenal chromaffin granules 1 . A similar enzyme is present in many brain regions 1,2 and in purified secretory granules from rat pituitary 3 and rat insulinoma 4 . Within the secretory granules, carboxypeptidase E (CPE) activity is found in both a soluble and a membrane-bound form 1 , which differ slightly in relative molecular mass ( M r ) 5 . Here, to investigate whether the CPE activities in the various tissues are produced from a single gene, purified CPE was partially sequenced and oligonucleotide probes were used to isolate a clone encoding CPE from a bovine pituitary complementary DNA library. This cDNA hybridizes to bovine pituitary poly(A) + RNAs of approximately 3.3, 2.6 and 2.1 kilobases (kb), with the 3.3-kb messenger RNA the predominant species. The predicted amino-acid sequence of the cDNA clone contains the partially determined sequences of CPE, several pairs of basic amino acids and displays some homology with both carboxypeptidases A and B. Restriction analysis of bovine genomic DNA suggests only one gene for CPE. This is consistent with a broad role for CPE in the biosynthesis of many neuropeptides.
ISSN:0028-0836
1476-4687
DOI:10.1038/323461a0