Use of the squirrel monkey Saimiri sciureus to optimize serological tests for diagnosis of Bunyavirus infection in humans

Several arthropod-borne viruses of the large Bunyaviridae virus family have been isolated in South America. There are few data about the incidence of these viruses in man, except for the Oropuche virus. Since haemagglutination inhibition tests are difficult to perform, only enzyme-linked immunosorbe...

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Bibliographic Details
Published in:Journal of virological methods 2001-04, Vol.93 (1), p.57-64
Main Authors: Morales-Betoulle, Maria Eugenia, Contamin, Hugues, Barnaud, Antoine, Talarmin, Antoine
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
Antibody responses
Biological and medical sciences
Bunyaviridae - immunology
Bunyaviridae Infections - blood
Bunyaviridae Infections - immunology
Bunyaviridae Infections - virology
Bunyavirus
Bunyaviruses
Disease Models, Animal
Experimental infection
Fundamental and applied biological sciences. Psychology
Immunoglobulin G - blood
Immunoglobulin M - blood
Kinetics
Microbiology
Neutralization Tests
Saimiri
Saimiri sciureus
Serological tests
South America
Viremia
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Summary:Several arthropod-borne viruses of the large Bunyaviridae virus family have been isolated in South America. There are few data about the incidence of these viruses in man, except for the Oropuche virus. Since haemagglutination inhibition tests are difficult to perform, only enzyme-linked immunosorbent assays (ELISAs) are used. Nevertheless, positive controls are necessary for ELISA, and infected humans are rare. Squirrel monkeys ( Saimiri sciureus) were therefore infected experimentally to assess their value as positive controls in such assays. The kinetics of viraemia and of antibody responses after infection with eight Bunyaviruses present in the Amazonian forest were studied. No viraemia was seen in most cases, but, with every virus studied, immunoglobulin (Ig)M and IgG antibody responses were observed, beginning between days 5 and 14 after infection for IgM and days 14–18 after infection for IgG. This model thus provides reliable positive controls for ELISAs in humans. Their availability will allow determination of the seroprevalence of Bunyaviruses in the human population of French Guiana.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(00)00295-0