Inhibitory effect of lead on the proliferation of cultured vascular endothelial cells

We investigated the effect of lead nitrate (0.5, 1.0, 2.0 or 5.0 μM) on the proliferation of cultured bovine aortic endothelial cells. After exposure to lead, the number of cells and the incorporation of [ 3H]thymidine into the acid-insoluble fraction of the cells were reduced in parallel in a conce...

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Published in:Toxicology (Amsterdam) 1995-01, Vol.95 (1), p.87-92
Main Authors: Kaji, Toshiyuki, Fujiwara, Yasuyuki, Hoshino, Miho, Yamamoto, Chika, Sakamoto, Michiko, Kozuka, Hiroshi
Format: Article
Language:English
Subjects:
Animals
Aorta - cytology
Biological and medical sciences
Cattle
Cell Division - drug effects
Cells, Cultured
Chemical and industrial products toxicology. Toxic occupational diseases
DNA - biosynthesis
DNA synthesis
Endothelial cells
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
Fibroblast growth factor
Fibroblast Growth Factor 1 - metabolism
Fibroblast Growth Factor 2 - metabolism
Lead
Lead - pharmacology
Medical sciences
Metals and various inorganic compounds
Nitrates - pharmacology
Proliferation
Thymidine - metabolism
Toxicology
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Summary:We investigated the effect of lead nitrate (0.5, 1.0, 2.0 or 5.0 μM) on the proliferation of cultured bovine aortic endothelial cells. After exposure to lead, the number of cells and the incorporation of [ 3H]thymidine into the acid-insoluble fraction of the cells were reduced in parallel in a concentration-dependent manner. Histologically, lead treatment resulted in a decrease in the cell number accompanied by a change in the cell shape from polygonal to spindle; however, no degenerative change was observed except in 5.0 μM lead-treated cells. Furthermore, stimulation of [ 3H]thymidine incorporation by either basic or acidic fibroblast growth factor was significantly reduced by lead. However, the leakage of lactate dehydrogenase into the medium from the cells, a marker of nonspecific cell damage, was not changed by lead. From these results, it was revealed that lead inhibits the proliferation of cultured vascular endothelial cells without nonspecific cell damage. Although lead does not destroy the monolayer of endothelial cells, the metal may exhibit its noxious effect in the repair process of the vascular endothelium.
ISSN:0300-483X
1879-3185
DOI:10.1016/0300-483X(94)02887-Z