Synthesis of Neoglycoproteins Using Oligosaccharide-transfer Activity with Endo-β-N-Acetylglucosaminidase

• Published in: The Journal of biological chemistry 1995-02, Vol.270 (7), p.3094-3099
• Main Authors: Takegawa, Kaoru, Tabuchi, Mitsuaki, Yamaguchi, Shinya, Kondo, Akihiro, Kato, Ikunoshin, Iwahara, Shojiro
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Arthrobacter - enzymology; Arthrobacter protophormiae; Carbohydrate Sequence; Chromatography, Affinity; Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Glycopeptides - chemical synthesis; Glycopeptides - isolation & purification; Glycoproteins - biosynthesis; Glycoproteins - chemical synthesis; Glycoproteins - isolation & purification; Indicators and Reagents; Kinetics; Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase - metabolism; Molecular Sequence Data; Oligosaccharides - metabolism; Ovalbumin; Ribonucleases - chemical synthesis; Ribonucleases - isolation & purification
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.270.7.3094

We describe a novel method for the enzymatic synthesis of neoglycoproteins. Endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) had high levels of transglycosylation activity. The enzyme activity of Endo-A was markedly increased by adding 4-L-aspartylglycosylamine (GlcNAc-Asn) to the reaction mixture. Digesting (Man)6(GlcNAc)2 with the enzyme in the presence of GlcNAc-Asn gave a mixture of hydrolytic ((Man)6GlcNAc) and transglycosylic ((Man)6(GlcNAc)2Asn) products. By means of transglycosylation, (Man)6GlcNAc was transferred en bloc to the partially deglycosylated ovalbumin glycopeptide (EEKYN(GlcNAc) LTSVL) concomitant with the hydrolysis of (Man)6(GlcNAc)2Asn. The structure of the transglycosylation product was designated as (Man)6(GlcNAc)2-peptide by amino acid composition and sequence analysis as well as ion mass spectrometry. The enzyme also transferred oligosaccharide to partially deglycosylated ribonuclease B (GlcNAc-protein) during the hydrolysis of (Man)6(GlcNAc)2Asn. Native ribonuclease B had (Man)5-9 (GlcNAc)2 as its heterogeneous N-linked sugar chains. High performance liquid chromatography showed that all of the N-linked sugar chains of the synthetic neoribonuclease of the pyridylamino derivatives were modified to (Man)6(GlcNAc)2.