Cloning and characterization of a mouse gene with homology to the human von Hippel-Lindau disease tumor suppressor gene : implications for the potential organization of the human von Hippel-Lindau disease gene

The human von Hippel-Lindau disease (VHL) gene has recently been identified and, based on the nucleotide sequence of a partial cDNA clone, has been predicted to encode a novel protein with as yet unknown functions [F. Latif et al., Science (Washington DC), 260: 1317-1320, 1993]. The length of the en...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1995-02, Vol.55 (4), p.743-747
Main Authors: JIZONG GAO, NAGLICH, J. G, LAIDLAW, J, WHALEY, J. M, SEIZINGER, B. R, KLEY, N
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Blotting, Northern
Cloning, Molecular
DNA Probes
DNA, Complementary - genetics
Genes, Tumor Suppressor
Humans
Medical sciences
Mice
Molecular Sequence Data
Neurology
Open Reading Frames
RNA, Messenger - genetics
Sequence Homology, Nucleic Acid
Tumors of the nervous system. Phacomatoses
von Hippel-Lindau Disease - genetics
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Summary:The human von Hippel-Lindau disease (VHL) gene has recently been identified and, based on the nucleotide sequence of a partial cDNA clone, has been predicted to encode a novel protein with as yet unknown functions [F. Latif et al., Science (Washington DC), 260: 1317-1320, 1993]. The length of the encoded protein and the characteristics of the cellular expressed protein are as yet unclear. Here we report the cloning and characterization of a mouse gene (mVHLh1) that is widely expressed in different mouse tissues and shares high homology with the human VHL gene. It predicts a protein 181 residues long (and/or 162 amino acids, considering a potential alternative start codon), which across a core region of approximately 140 residues displays a high degree of sequence identity (98%) to the predicted human VHL protein. High stringency DNA and RNA hybridization experiments and protein expression analyses indicate that this gene is the most highly VHL-related mouse gene, suggesting that it represents the mouse VHL gene homologue rather than a related gene sharing a conserved functional domain. These findings provide new insights into the potential organization of the VHL gene and nature of its encoded protein.
ISSN:0008-5472
1538-7445