Absence of Infectious Retinitis after Injection of Human Cytomegalovirus into Rabbit Eyes

The rabbit model of human cytomegalovirus (HCMV) retinitis was evaluated by preretinal and intravitreal injection of HCMV into rabbit eyes. Ocular disease was evaluated by indirect ophthalmoscopy, histopathology, and immunohistochemistry. Vitritis, optic nerve congestion, multiple small white infilt...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1995-04, Vol.171 (4), p.782-787
Main Authors: Tatebayashi, Misako, Neyts, Johan, Besen, Gilberto, Flores-Aguilar, Marisa, Smith, Irene L., Wiley, Clayton A., Spector, Stephen A., Lynn, Germaine Bergeron, Maudgal, Prabhat C., Clercq, Erik De, Gangan, Pacifico A., Chavez, Eugenio, Russack, Valentina, Freeman, William R.
Format: Article
Language:English
Subjects:
Animals
Antigens, Viral - analysis
Culture Media
Cytomegalovirus
Cytomegalovirus - immunology
Cytomegalovirus - pathogenicity
Cytomegalovirus infections
Cytomegalovirus Retinitis - pathology
Cytomegalovirus Retinitis - virology
Disease Models, Animal
Eyes
Humans
Infections
Inoculation
Major Articles
Optic nerve
Rabbits
Retina
Retina - pathology
Retina - virology
Retinitis
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The rabbit model of human cytomegalovirus (HCMV) retinitis was evaluated by preretinal and intravitreal injection of HCMV into rabbit eyes. Ocular disease was evaluated by indirect ophthalmoscopy, histopathology, and immunohistochemistry. Vitritis, optic nerve congestion, multiple small white infiltrates in the cortical vitreous or on the retinal surface, and retinal detachments were seen. Histopathologic examination showed inflammatory cell infiltration in the preretinal vitreous, optic nerve, and transiently in the superficial inner retina. Retinal structure was preserved except for changes in areas of retinal detachment. No necrosis or destruction of the retina was seen. Immunohistochemistry showed no evidence of cytomegalovirus infection. Inoculation of culture medium containing fetal calf serum caused a similar reaction. It is concluded that vitreous and retinal inoculation of HCMV in the rabbit eye caused nonspecific inflammation without evidence of infection, so this is not a suitable model for HCMV retinitis.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/171.4.782