Difficulties in obtaining monoclonal antibodies to subsets of human leukocytes, using neonatal tolerance induction in mice

• Published in: Journal of immunological methods 1995-04, Vol.181 (1), p.115-124
• Main Authors: Yamaguchi, Yasunori, O'Doherty, Una, Peng, Michael, Steinman, Ralph M.
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn - immunology; Antibodies, immunoglobulins; Antibodies, Monoclonal - isolation & purification; Biological and medical sciences; Dendritic cell; Dendritic Cells - immunology; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immune Tolerance; Immunization; Lymphocyte Activation - immunology; Lymphocyte Subsets - immunology; Lymphocyte Subsets - transplantation; Male; Mice; Mice, Inbred BALB C; Mice, Inbred DBA; Molecular immunology; Monoclonal antibodies; Monoclonal antibody; Neonatal tolerance; Pregnancy; Selective antibody response
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/0022-1759(94)00335-T

Many antigens are shared between different types of human leukocytes. In an effort to obtain new lineage-specific monoclonal antibodies, particularly antibodies to dendritic cells, we attempted to tolerize newborn mice to one type of leukocyte and then immunize the adults with another. We found that T cells, either unstimulated T cells or T blasts, were more effective at inducing neonatal tolerance than non-T cells or B cell lines. However, the tolerance that was achieved was not restricted to T cells, since we could not elicit from the tolerized mice a specific antibody response to a B cell line or to blood dendritic cells. Here we describe several efforts, all unsuccessful, to achieve cell specific immune responses in tolerant mice. The parameters we considered included the type of cell used to tolerize neonatal mice, the regimen of injections for inducing tolerance or eliciting immunity, and the use of several different adjuvants.