Evolution of the Phycobiliproteins

• Published in: Journal of molecular biology 1995-04, Vol.248 (1), p.79-96
• Main Authors: Apt, Kirk E., Collier, Jackie L., Grossman, Arthur R.
• Format: Article
• Language: English
• Subjects: allophycocyanin; allophycocyanins; Amino Acid Sequence; amino acid sequence alignment; amino acid sequences; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Biological Evolution; Consensus Sequence; Cyanobacteria; Cyanobacteria - genetics; Cyanophyta; Eukaryotic Cells; Freshwater; light harvesting complex; Light-Harvesting Protein Complexes; Macromolecular Substances; Molecular Sequence Data; Phycobilisomes; phycocyanin; phycocyanins; phycoerythrin; phycoerythrins; Phylogeny; Plant Proteins - chemistry; Plant Proteins - genetics; Protein Conformation; proteins; red algae; Rhodophycota; Sequence Homology, Amino Acid; Space life sciences; Synechococcus
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1006/jmbi.1995.0203

Amino acid sequence alignments and phylogenetic analyses have been used to examine the relationships among 100 phycobiliprotein sequences. The alignments revealed a number of highly conserved amino acid residues that are involved in chromophore attachment and conformation, α-β interactions and phycobilisome assembly. The phylogenetic analysis confirmed that the phycobiliprotein subfamilies, previously classified by their biochemical and spectroscopic properties, also formed coherent evolutionary groups. The α and β subunits formed two distinct evolutionary lines that originate from a common ancestor. The pattern of divergence among the α subfamilies was identical to that of the β subfamilies, strongly suggesting that the α and β subunits of each phycobiliprotein type have coevolved. The phylogenetic data support a monophyletic separation of the eukaryotic sequences from the extant cyanobacterial sequences. The eukaryotic phycoerythrins appeared more closely related to the marine Synechococcusphycoerythrins than to the other cyanobacterial phycoerythrins. The cryptophyte phycobiliproteins formed a monophyletic group within the rhodophyte lineage. In conjunction with other phylogenetic markers, the analysis of additional phycobiliprotein sequences may help to further resolve the relationships among phycobiliprotein-containing organisms.