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PCNA-Immunoreactivity in the uterus of rats after treatment with the antiestrogen Tamoxifen
We used an antibody to the proliferating cell nuclear antigen (PCNA) to investigate the effect of the long-term administration of tamoxifen on proliferative activity in the uterus of mature rats. Untreated cycling and ovariectomized rats served as controls. The PCNA labelling indices (PI) and the mi...
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Published in: | Annals of anatomy 1995-03, Vol.177 (2), p.133-138 |
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container_title | Annals of anatomy |
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creator | Rumpel, Elisabeth Michna, Horst Kühnel, Wolfgang |
description | We used an antibody to the proliferating cell nuclear antigen (PCNA) to investigate the effect of the long-term administration of tamoxifen on proliferative activity in the uterus of mature rats. Untreated cycling and ovariectomized rats served as controls. The PCNA labelling indices (PI) and the mitotic indices (MI) were estimated for the luminal and glandular epithelium and for the stromal fibroblasts. A strong correlation was found for PI and MI in the luminal and in the glandular epithelium, and a lower, but also significant correlation, for the endometrial stroma cells. Tamoxifen treatment decreased the PI of the luminal epithelial cells and of the stroma as much as ovariectomy. In both of these groups, the proportion of anti-PCNA positive cells in the glandular epithelium was significantly higher than in the luminal epithelium. These data indicate that tamoxifen has a strong antiproliferative effect on the uterus of mature rats, and that this antiestrogenic action is cell type specific. |
doi_str_mv | 10.1016/S0940-9602(11)80060-9 |
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These data indicate that tamoxifen has a strong antiproliferative effect on the uterus of mature rats, and that this antiestrogenic action is cell type specific.</description><subject>Animals</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Epithelial Cells</subject><subject>Epithelium - drug effects</subject><subject>Estrus - physiology</subject><subject>Female</subject><subject>Immunohistochemistry</subject><subject>Ovariectomy</subject><subject>PCNA immunohistochemistry</subject><subject>Proliferating Cell Nuclear Antigen - analysis</subject><subject>Proliferation</subject><subject>Rat uterus</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reference Values</subject><subject>Tamoxifen</subject><subject>Tamoxifen - pharmacology</subject><subject>Uterus - cytology</subject><subject>Uterus - drug effects</subject><issn>0940-9602</issn><issn>1618-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqFkEtLAzEQgIMotVZ_QiEn0cNqkt1kNycpxUehqGA9eQhpdtZGullNstX-e7cPvHoahvnm9SE0pOSKEiquX4jMSCIFYReUXhaEiC47QH0qaJGQjLBD1P9DjtFJCB-EpIKLrId6eZ5RlrM-enseP46SSV23rvGgTbQrG9fYOhwXgNsIvg24qbDXMWBddTmOHRdrcBF_27jYctpFCyH65h0cnum6-bEVuFN0VOllgLN9HKDXu9vZ-CGZPt1PxqNpYlJBYqJByjylrOCaSpExIVJDJRVFYQAEn-dlxSBPGRGSVxxEOucgeVZCVhbaFDQdoPPd3E_ffLXdHaq2wcByqR00bVB5zjgvJO9AvgONb0LwUKlPb2vt14oStZGqtlLVxpiiVG2lKtn1DfcL2nkN5V_X3mJXv9nVoftyZcGrYCw4A6X1YKIqG_vPhl8sY4bB</recordid><startdate>19950301</startdate><enddate>19950301</enddate><creator>Rumpel, Elisabeth</creator><creator>Michna, Horst</creator><creator>Kühnel, Wolfgang</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19950301</creationdate><title>PCNA-Immunoreactivity in the uterus of rats after treatment with the antiestrogen Tamoxifen</title><author>Rumpel, Elisabeth ; Michna, Horst ; Kühnel, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-ae99731285a19642663c191688cee65b7df2e7320695f5e63b5e954de4d8ac813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Epithelial Cells</topic><topic>Epithelium - drug effects</topic><topic>Estrus - physiology</topic><topic>Female</topic><topic>Immunohistochemistry</topic><topic>Ovariectomy</topic><topic>PCNA immunohistochemistry</topic><topic>Proliferating Cell Nuclear Antigen - analysis</topic><topic>Proliferation</topic><topic>Rat uterus</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reference Values</topic><topic>Tamoxifen</topic><topic>Tamoxifen - pharmacology</topic><topic>Uterus - cytology</topic><topic>Uterus - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rumpel, Elisabeth</creatorcontrib><creatorcontrib>Michna, Horst</creatorcontrib><creatorcontrib>Kühnel, Wolfgang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of anatomy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rumpel, Elisabeth</au><au>Michna, Horst</au><au>Kühnel, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PCNA-Immunoreactivity in the uterus of rats after treatment with the antiestrogen Tamoxifen</atitle><jtitle>Annals of anatomy</jtitle><addtitle>Ann Anat</addtitle><date>1995-03-01</date><risdate>1995</risdate><volume>177</volume><issue>2</issue><spage>133</spage><epage>138</epage><pages>133-138</pages><issn>0940-9602</issn><eissn>1618-0402</eissn><abstract>We used an antibody to the proliferating cell nuclear antigen (PCNA) to investigate the effect of the long-term administration of tamoxifen on proliferative activity in the uterus of mature rats. 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source | ScienceDirect Journals |
subjects | Animals Cell Nucleus - drug effects Cell Nucleus - ultrastructure Epithelial Cells Epithelium - drug effects Estrus - physiology Female Immunohistochemistry Ovariectomy PCNA immunohistochemistry Proliferating Cell Nuclear Antigen - analysis Proliferation Rat uterus Rats Rats, Sprague-Dawley Reference Values Tamoxifen Tamoxifen - pharmacology Uterus - cytology Uterus - drug effects |
title | PCNA-Immunoreactivity in the uterus of rats after treatment with the antiestrogen Tamoxifen |
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