Transcriptional Regulation of Phospholamban Gene and Translational Regulation of SERCA2 Gene Produces Coordinate Expression of These Two Sarcoplasmic Reticulum Proteins during Skeletal Muscle Phenotype Switching

Chronic 1 Hz stimulation of the canine latissimus dorsi muscle produced a time-dependent switch from the fast-twitch to the slow-twitch phenotype. This included changes in the proteins of the sarcoplasmic reticulum. After 3 days of muscle stimulation, there was down-regulation of fast-twitch Ca-ATPa...

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Published in:The Journal of biological chemistry 1995-05, Vol.270 (19), p.11619-11622
Main Authors: Hu, Ping, Yin, Chang, Zhang, Ke-Min, Wright, Leon D., Nixon, Todd E., Wechsler, Andrew S., Spratt, John A., Briggs, F. Norman
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - biosynthesis
Animals
Calcium-Binding Proteins - biosynthesis
Calcium-Transporting ATPases - biosynthesis
Conditioning, Psychological
Dogs
Electric Stimulation
Gene Expression Regulation, Enzymologic
Microsomes - enzymology
Muscle Fibers, Fast-Twitch - physiology
Muscle Fibers, Slow-Twitch - physiology
Muscle, Skeletal - enzymology
Muscle, Skeletal - physiology
Phenotype
Protein Biosynthesis
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Sarcoplasmic Reticulum - enzymology
Space life sciences
Time Factors
Transcription, Genetic
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Summary:Chronic 1 Hz stimulation of the canine latissimus dorsi muscle produced a time-dependent switch from the fast-twitch to the slow-twitch phenotype. This included changes in the proteins of the sarcoplasmic reticulum. After 3 days of muscle stimulation, there was down-regulation of fast-twitch Ca-ATPase (SERCA1a) mRNA and induction of slow-twitch Ca-ATPase (SERCA2a) mRNA; most changes in both mRNAs were nearly complete after 14 days of stimulation. Although the induction of phospholamban mRNA began after 3 days of muscle stimulation, its up-regulation was not completed until the muscle had been stimulated for 42 days. The time course of expression of SERCA2a protein was very different from that of SERCA2a mRNA, suggesting that SERCA2 gene expression is regulated at the translational as well as the transcriptional level. The time course of expression of phospholamban protein closely followed that of phospholamban mRNA, suggesting that this gene is under transcriptional control. Thus coordinated expression of SERCA2a and phospholamban proteins is achieved via translational control of the SERCA2 gene and transcriptional control of the phospholamban gene.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.270.19.11619