Use of mucin and hemoglobin in experimental murine gram-negative bacteremia enhances the immunoprotective action of antibodies reactive with the lipopolysaccharide core region

An antiserum with a high content of antibodies, binding to the Gram-negative lipopolysaccharide core region, was prepared by immunizing rabbits with the rough Escherichia coli mutant J5. This antiserum was capable of protecting mice against lethal challenge doses of E. coli 0 111:B4 in a mouse model...

Full description

Saved in:
Bibliographic Details
Published in:Antonie van Leeuwenhoek 1986-01, Vol.52 (6), p.537-542
Main Authors: Appelmelk, B J, Verwey-van Vught, A M, Maaskant, J J, Schouten, W F, Thijs, L G, Maclaren, D M
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - immunology
Cross Reactions
Escherichia coli
Escherichia coli - immunology
Escherichia coli Infections - immunology
Female
Hemoglobins - immunology
Immune Sera - immunology
Immunization, Passive
Lipopolysaccharides - immunology
Mice
Mucins - immunology
Sepsis - immunology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An antiserum with a high content of antibodies, binding to the Gram-negative lipopolysaccharide core region, was prepared by immunizing rabbits with the rough Escherichia coli mutant J5. This antiserum was capable of protecting mice against lethal challenge doses of E. coli 0 111:B4 in a mouse model where the animals were compromised by means of mucin plus hemoglobin (LD 50 = 10(3) bacteria). However, no protection was observed in a non-compromised mouse model (LD 50 = 10(7) bacteria). This observation might explain why in the past so many discrepant results have been obtained in mouse protection studies with cross-reactive antisera.
ISSN:0003-6072
1572-9699
DOI:10.1007/BF00423414