Comparison of rapid diagnostic methodologies for chlamydia and gonorrhea in an urban adolescent population: A pilot study

To compare a combination DNA probe test which detects both N. gonorrhoeae (GC) and C. trachomatis (CT) to the current culture methodologies among a population of female adolescents at an urban teaching center. In addition, the probe test for CT was compared to a direct immunofluorescence test. All s...

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Bibliographic Details
Published in:Journal of adolescent health 1995-04, Vol.16 (4), p.324-327
Main Authors: Bryant, D.K., Fox, A.S., Spigland, I., Childers, E., Motyl, M., Rosenfeld, W.D.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Bacteriological Techniques
Biological and medical sciences
C. trachomatis
Chlamydia trachomatis - growth & development
Chlamydia trachomatis - isolation & purification
DNA probe test N. gonorrhoeae
DNA Probes
Female
General aspects
Human infectious diseases. Experimental studies and models
Humans
Infectious diseases
Medical sciences
Neisseria gonorrhoeae - growth & development
Neisseria gonorrhoeae - isolation & purification
Pilot Projects
Sensitivity and Specificity
Sexually transmitted diseases
Tissue culture test
Urban Population
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Summary:To compare a combination DNA probe test which detects both N. gonorrhoeae (GC) and C. trachomatis (CT) to the current culture methodologies among a population of female adolescents at an urban teaching center. In addition, the probe test for CT was compared to a direct immunofluorescence test. All sexually active female adolescents between the ages of 13–21 years who sought care at an urban teaching center from June 1991 through November 1991 and who required testing for sexually transmitted diseases (STDs) were recruited for this study. The probe test was demonstrated to be 66.6% sensitive and 94.9% specific when compared to tissue culture for CT and 50% sensitive and 98.2% specific when compared to culture for GC. We found an overall prevalence of 23.5% for CT and 3.5% for GC. The two rapid diagnostic tests for CT evaluated in this study demonstrated similar sensitivities. However the probe test offers advantages in that it is easier to perform, skill at reading fluorescence is not required, and one specimen yields results for both CT and GC.
ISSN:1054-139X
1879-1972
DOI:10.1016/1054-139X(94)00075-P