The rabbit muscle phosphofructokinase gene. Implications for protein structure, function, and tissue specificity

Sequence homologies between bacterial and rabbit muscle phosphofructokinases and between the amino- and carboxyl-terminal halves of the latter suggest that the mammalian enzyme evolved from a prokaryotic progenitor by gene duplication and divergence (Poorman, R. A., Randolph, A., Kemp, R. G., and He...

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Bibliographic Details
Published in:The Journal of biological chemistry 1987-03, Vol.262 (9), p.4195-4199
Main Authors: Lee, C.P., Kao, M.C., French, B.A., Putney, S.D., Chang, S.H.
Format: Article
Language:English
Subjects:
6-phosphofructokinase
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Biological Evolution
DNA
DNA, Recombinant
Escherichia coli - enzymology
Exons
Fundamental and applied biological sciences. Psychology
gene products
genes
Genes. Genome
Geobacillus stearothermophilus - enzymology
Introns
Molecular and cellular biology
Molecular genetics
Muscles - enzymology
nucleotide sequence
Phosphofructokinase-1 - genetics
Rabbits
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Summary:Sequence homologies between bacterial and rabbit muscle phosphofructokinases and between the amino- and carboxyl-terminal halves of the latter suggest that the mammalian enzyme evolved from a prokaryotic progenitor by gene duplication and divergence (Poorman, R. A., Randolph, A., Kemp, R. G., and Heinrikson, R. L. (1984) Nature 309, 467-469). We have isolated the gene for the rabbit enzyme and determined the nucleotide sequence for all the exons and most of the introns. This represents the first eukaryotic phosphofructokinase gene ever sequenced. The cloned gene is 17 kilobase pairs long. The coding sequence for 780 amino acids is split into 22 exons ranging in size from 15 to 63 codons. Sequence analysis shows that 75% of the bases at the third position of the codons in these exons are either G or C. Exons XV and XVI code for the 30 amino acid residues which were left unidentified in the published primary structure for this enzyme. When overlaid on the structure of the protein, most of the introns are located between or near the ends of the secondary structural elements but not at analogous positions in the two protein-coding halves of the gene.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)61332-8