As in Saccharomyces cerevisiae, aspartate transcarbamoylase is assembled on a multifunctional protein including a dihydroorotase-like cryptic domain in Schizosaccharomyces pombe

The organisation of the URA1 gene of Schizosaccharomyces pombe was determined from the entire cDNA cloned by the transformation of an ATCase-deficient strain of Saccharomyces cerevisiae. The URA1 gene encodes the bifunctional protein GLNase/CPSase-ATCase which catalyses the first two steps of the py...

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Bibliographic Details
Published in:Current genetics 1995-07, Vol.28 (2), p.138-149
Main Authors: Lollier, M, Jaquet, L, Nedeva, T, Lacroute, F, Potier, S, Souciet, J.L. (Strasbourg Univ., Centre National de la Recherche Scientifique (France). Lab. de Microbiologie et de Genetique)
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Anthranilate Synthase
Aspartate Carbamoyltransferase - antagonists & inhibitors
Aspartate Carbamoyltransferase - genetics
Aspartate Carbamoyltransferase - metabolism
Base Sequence
BIOSINTESIS
BIOSYNTHESE
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) - antagonists & inhibitors
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) - genetics
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) - metabolism
Cloning, Molecular
DHOase-like
Dihydroorotase - metabolism
DNA, Fungal
Feedback inhibition
GENE
GENES
INHIBICION
INHIBITION
Molecular Sequence Data
Multienzyme Complexes - antagonists & inhibitors
Multienzyme Complexes - genetics
Multienzyme Complexes - metabolism
Multifunctional protein
Nitrogenous Group Transferases
Pflanzenzuechtung
PIRIMIDINAS
PYRIMIDINE
Pyrimidine pathway
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Schizosaccharomyces - enzymology
Schizosaccharomyces - genetics
SCHIZOSACCHAROMYCES POMBE
SECUENCIA NUCLEOTIDICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEOTIDIQUE
Transferases - metabolism
Uridine Triphosphate - metabolism
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Description
Summary:The organisation of the URA1 gene of Schizosaccharomyces pombe was determined from the entire cDNA cloned by the transformation of an ATCase-deficient strain of Saccharomyces cerevisiae. The URA1 gene encodes the bifunctional protein GLNase/CPSase-ATCase which catalyses the first two steps of the pyrimidine biosynthesis pathway. The complete nucleotide sequence of the URA1 cDNA was elucidated and the deduced amino-acid sequence was used to define four domains in the protein; three functional domains, corresponding to GLNase (glutamine amidotransferase), CPSase (carbamoylphosphate synthetase) and ATCase (aspartate transcarbamoylase) activities, and one cryptic DHOase (dihydroorotase) domain. Genetic investigations confirmed that both GLNase/CPSase and ATCase activities are carried out by the same polypeptide. They are also both feedbackinhibited by UTP (uridine triphosphate). Its organization and regulation indicate that the S. pombe URA1 gene product appears very similar to the S. cerevisiae URA2 gene product.
ISSN:0172-8083
1432-0983
DOI:10.1007/bf00315780