Effect of mitogens on the cell cycle progression and the quantification of T-lymphocyte surface markers in acquired immune deficiency syndrome

The cell cycle progression and viability of stimulated and intact lymphocytes from 20 subjects with acquired immune deficiency syndrome (AIDS) was determined by flow cytometry. As compared to controls, 62% less AIDS lymphocytes, cultured for 72 hr in the presence of lectins (Con‐A, PHA, PWM), had en...

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Bibliographic Details
Published in:Journal of leukocyte biology 1987-08, Vol.42 (2), p.122-127
Main Authors: Hornicek, F.J., Malinin, G.I., Thornthwaite, J.T., Whiteside, M.E., MacLeod, C.L., Malinin, T.I.
Format: Article
Language:English
Subjects:
acquired immune deficiency syndrome
Acquired Immunodeficiency Syndrome - immunology
AIDS
AIDS/HIV
Antigens, Surface - analysis
Biological and medical sciences
Cell Cycle - drug effects
cell surface
cytometry
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Interleukin-2 - biosynthesis
Lymphocyte Activation
lymphocytes
lymphocytes T
Male
man
Medical sciences
mitogens
Phenotype
Phytohemagglutinins - pharmacology
T-Lymphocytes - classification
T-Lymphocytes - immunology
viability
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Summary:The cell cycle progression and viability of stimulated and intact lymphocytes from 20 subjects with acquired immune deficiency syndrome (AIDS) was determined by flow cytometry. As compared to controls, 62% less AIDS lymphocytes, cultured for 72 hr in the presence of lectins (Con‐A, PHA, PWM), had entered the proliferative phases of the cell cycle, while the respective value for periodic‐acid (H5lO6)‐stimulated cells was 34%. The helper‐suppressor ratios and natural kill cell percentages of the unstimulated and PHA‐activated AIDS lymphocytes increased approximately 3‐fold after 72 hr in culture. The natural killer (NK) cell fraction of the PHA‐stimulated and unstimulated AIDS cultures comprised approximately 20% as compared to 10% in controls. However, no changes in the percentages of T‐lymphocytes were detected in the AIDS cell cultures. Throughout the culture period, viability of the unstimulated AIDS lymphocytes exceeded 90%, whereas in stimulated cultures it fluctuated within the 65‐90% range. It is concluded that the liability of AIDS lymphocytes to mitogens is probably a direct consequence of the human immunodeficiency virus (HIV) infection.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.42.2.122