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Pathogenesis of foot-and-mouth disease in swine, studied by in-situ hybridization

Eight 7-month-old pigs were inoculated intradermally with 10 3 plaqueforming units of foot-and-mouth disease virus, type O, and killed 24, 48, 72, or 96 h later. Numerous tissues from each animal were collected and examined histopathologically and by in-situ hybridization to determine the presence o...

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Bibliographic Details
Published in:Journal of comparative pathology 1995-07, Vol.113 (1), p.51-58
Main Authors: Brown, C.C., Olander, H.J., Meyer, R.F.
Format: Article
Language:English
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Summary:Eight 7-month-old pigs were inoculated intradermally with 10 3 plaqueforming units of foot-and-mouth disease virus, type O, and killed 24, 48, 72, or 96 h later. Numerous tissues from each animal were collected and examined histopathologically and by in-situ hybridization to determine the presence of virus and its correlation with lesion development. The probe for in-situ hybridization was a biotinylated 500-base negative-sense transcription product corresponding to a portion of the gene encoding polymerase. With this technique, virus was shown to be widely disseminated in all epidermal tissues, regardless of histologically apparent cellular disruption.
ISSN:0021-9975
1532-3129
DOI:10.1016/S0021-9975(05)80068-4