Loading…
Pathogenesis of foot-and-mouth disease in swine, studied by in-situ hybridization
Eight 7-month-old pigs were inoculated intradermally with 10 3 plaqueforming units of foot-and-mouth disease virus, type O, and killed 24, 48, 72, or 96 h later. Numerous tissues from each animal were collected and examined histopathologically and by in-situ hybridization to determine the presence o...
Saved in:
Published in: | Journal of comparative pathology 1995-07, Vol.113 (1), p.51-58 |
---|---|
Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Eight 7-month-old pigs were inoculated intradermally with 10
3 plaqueforming units of foot-and-mouth disease virus, type O, and killed 24, 48, 72, or 96 h later. Numerous tissues from each animal were collected and examined histopathologically and by in-situ hybridization to determine the presence of virus and its correlation with lesion development. The probe for in-situ hybridization was a biotinylated 500-base negative-sense transcription product corresponding to a portion of the gene encoding polymerase. With this technique, virus was shown to be widely disseminated in all epidermal tissues, regardless of histologically apparent cellular disruption. |
---|---|
ISSN: | 0021-9975 1532-3129 |
DOI: | 10.1016/S0021-9975(05)80068-4 |