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Bimodal distribution of proteinase 3 (PR3) surface expression reflects a constitutive heterogeneity in the polymorphonuclear neutrophil pool
Proteinase 3, which is known as an intracellular serine protease of neutrophils, was detected at the surface of a subpopulation of freshly isolated PMN. The proportion of PR3-positive and -negative PMN, observed by flow cytometry with anti-PR3 mAbs or ANCA autoantibodies, varies among individuals bu...
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Published in: | FEBS letters 1995-10, Vol.374 (1), p.29-33 |
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creator | Halbwachs-Mecarelli, L. Bessou, G. Lesavre, P. Lopez, S. Witko-Sarsat, V. |
description | Proteinase 3, which is known as an intracellular serine protease of neutrophils, was detected at the surface of a subpopulation of freshly isolated PMN. The proportion of PR3-positive and -negative PMN, observed by flow cytometry with anti-PR3 mAbs or ANCA autoantibodies, varies among individuals but is extremely stable for each individual over prolonged time periods. After PMN degranulation by FMLP with cyt. B, membrane PR3 expression increases but the proportion of low and high PR3-expressing cells remains stable. The existence of a subset of PMN which spontaneously expresses PR3 and varies among individuals, may be relevant to the pathogenesis of anti-PR3 ANCA autoantibody-related vasculitis. |
doi_str_mv | 10.1016/0014-5793(95)01073-N |
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The proportion of PR3-positive and -negative PMN, observed by flow cytometry with anti-PR3 mAbs or ANCA autoantibodies, varies among individuals but is extremely stable for each individual over prolonged time periods. After PMN degranulation by FMLP with cyt. B, membrane PR3 expression increases but the proportion of low and high PR3-expressing cells remains stable. 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B, cytochalasin B ; fMLP, N-formyl-methionyl-leucyl-phenylalanine ; Granulomatosis with Polyangiitis - immunology ; HBSS, Hanks' balanced salt solution ; Humans ; Mice ; Myeloblastin ; N-Formylmethionine Leucyl-Phenylalanine ; Neutrophils - enzymology ; Neutrophils - immunology ; Phenotype ; PMN serine protease ; PMN, polymorphonuclear neutrophils ; Polymorphonuclear neutrophil ; PR3, proteinase 3 ; Proteinase 3 ; Serine Endopeptidases - classification ; Serine Endopeptidases - metabolism ; Vasculitis ; WG, Wegener's granulomatosis</subject><ispartof>FEBS letters, 1995-10, Vol.374 (1), p.29-33</ispartof><rights>1995</rights><rights>FEBS Letters 374 (1995) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c583N-291eeb24ea37a97d2e22b489705d6dff2a6bf0e926401afaf249e2cd4d9d45eb3</citedby><cites>FETCH-LOGICAL-c583N-291eeb24ea37a97d2e22b489705d6dff2a6bf0e926401afaf249e2cd4d9d45eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001457939501073N$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7589506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Halbwachs-Mecarelli, L.</creatorcontrib><creatorcontrib>Bessou, G.</creatorcontrib><creatorcontrib>Lesavre, P.</creatorcontrib><creatorcontrib>Lopez, S.</creatorcontrib><creatorcontrib>Witko-Sarsat, V.</creatorcontrib><title>Bimodal distribution of proteinase 3 (PR3) surface expression reflects a constitutive heterogeneity in the polymorphonuclear neutrophil pool</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Proteinase 3, which is known as an intracellular serine protease of neutrophils, was detected at the surface of a subpopulation of freshly isolated PMN. The proportion of PR3-positive and -negative PMN, observed by flow cytometry with anti-PR3 mAbs or ANCA autoantibodies, varies among individuals but is extremely stable for each individual over prolonged time periods. After PMN degranulation by FMLP with cyt. B, membrane PR3 expression increases but the proportion of low and high PR3-expressing cells remains stable. The existence of a subset of PMN which spontaneously expresses PR3 and varies among individuals, may be relevant to the pathogenesis of anti-PR3 ANCA autoantibody-related vasculitis.</description><subject>ANCA</subject><subject>ANCA, antineutrophil cytoplasmic autoantibodies</subject><subject>Animals</subject><subject>Arthritis, Rheumatoid - immunology</subject><subject>Autoantibodies - immunology</subject><subject>Blood Donors</subject><subject>Cell Membrane - enzymology</subject><subject>cyt. B, cytochalasin B</subject><subject>fMLP, N-formyl-methionyl-leucyl-phenylalanine</subject><subject>Granulomatosis with Polyangiitis - immunology</subject><subject>HBSS, Hanks' balanced salt solution</subject><subject>Humans</subject><subject>Mice</subject><subject>Myeloblastin</subject><subject>N-Formylmethionine Leucyl-Phenylalanine</subject><subject>Neutrophils - enzymology</subject><subject>Neutrophils - immunology</subject><subject>Phenotype</subject><subject>PMN serine protease</subject><subject>PMN, polymorphonuclear neutrophils</subject><subject>Polymorphonuclear neutrophil</subject><subject>PR3, proteinase 3</subject><subject>Proteinase 3</subject><subject>Serine Endopeptidases - classification</subject><subject>Serine Endopeptidases - metabolism</subject><subject>Vasculitis</subject><subject>WG, Wegener's granulomatosis</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqNUdGO1CAUJUazjqt_oAlPZvehChTa8mLibnbUZDMao8-Ewq2DoaUCXZ1_8KOlzmQfjU_APeeeyz0HoeeUvKKENq8JobwSrawvpLgklLR1tXuANrQrl5o33UO0uac8Rk9S-k7Ku6PyDJ21opOCNBv0-8qNwWqPrUs5un7JLkw4DHiOIYObdAJc44tPn-tLnJY4aAMYfs0RUlqJEQYPJiessQlTyi4XgTvAe8gQwzeYwOUDdhPOe8Bz8IcxxHkfpsV40BFPsOQY5r3zBQz-KXo0aJ_g2ek8R1-3N1-u31e3H999uH57WxnR1buKSQrQMw66brVsLQPGet7Jlgjb2GFguukHApI1nFA96IFxCcxYbqXlAvr6HL086pYlfyyQshpdMuC9niAsSbVt6RRCFiI_Ek0MKZVl1RzdqONBUaLWENTqsFodVlKovyGoXWl7cdJf-hHsfdPJ9YJvj_hP5-HwX5pqe3PFVmCtF5G1ug56cxSC4tadg6iScTAZsC6WWJQN7t8__QOcWa37</recordid><startdate>19951023</startdate><enddate>19951023</enddate><creator>Halbwachs-Mecarelli, L.</creator><creator>Bessou, G.</creator><creator>Lesavre, P.</creator><creator>Lopez, S.</creator><creator>Witko-Sarsat, V.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19951023</creationdate><title>Bimodal distribution of proteinase 3 (PR3) surface expression reflects a constitutive heterogeneity in the polymorphonuclear neutrophil pool</title><author>Halbwachs-Mecarelli, L. ; Bessou, G. ; Lesavre, P. ; Lopez, S. ; Witko-Sarsat, V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c583N-291eeb24ea37a97d2e22b489705d6dff2a6bf0e926401afaf249e2cd4d9d45eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>ANCA</topic><topic>ANCA, antineutrophil cytoplasmic autoantibodies</topic><topic>Animals</topic><topic>Arthritis, Rheumatoid - immunology</topic><topic>Autoantibodies - immunology</topic><topic>Blood Donors</topic><topic>Cell Membrane - enzymology</topic><topic>cyt. B, cytochalasin B</topic><topic>fMLP, N-formyl-methionyl-leucyl-phenylalanine</topic><topic>Granulomatosis with Polyangiitis - immunology</topic><topic>HBSS, Hanks' balanced salt solution</topic><topic>Humans</topic><topic>Mice</topic><topic>Myeloblastin</topic><topic>N-Formylmethionine Leucyl-Phenylalanine</topic><topic>Neutrophils - enzymology</topic><topic>Neutrophils - immunology</topic><topic>Phenotype</topic><topic>PMN serine protease</topic><topic>PMN, polymorphonuclear neutrophils</topic><topic>Polymorphonuclear neutrophil</topic><topic>PR3, proteinase 3</topic><topic>Proteinase 3</topic><topic>Serine Endopeptidases - classification</topic><topic>Serine Endopeptidases - metabolism</topic><topic>Vasculitis</topic><topic>WG, Wegener's granulomatosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Halbwachs-Mecarelli, L.</creatorcontrib><creatorcontrib>Bessou, G.</creatorcontrib><creatorcontrib>Lesavre, P.</creatorcontrib><creatorcontrib>Lopez, S.</creatorcontrib><creatorcontrib>Witko-Sarsat, V.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Halbwachs-Mecarelli, L.</au><au>Bessou, G.</au><au>Lesavre, P.</au><au>Lopez, S.</au><au>Witko-Sarsat, V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bimodal distribution of proteinase 3 (PR3) surface expression reflects a constitutive heterogeneity in the polymorphonuclear neutrophil pool</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1995-10-23</date><risdate>1995</risdate><volume>374</volume><issue>1</issue><spage>29</spage><epage>33</epage><pages>29-33</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Proteinase 3, which is known as an intracellular serine protease of neutrophils, was detected at the surface of a subpopulation of freshly isolated PMN. The proportion of PR3-positive and -negative PMN, observed by flow cytometry with anti-PR3 mAbs or ANCA autoantibodies, varies among individuals but is extremely stable for each individual over prolonged time periods. After PMN degranulation by FMLP with cyt. B, membrane PR3 expression increases but the proportion of low and high PR3-expressing cells remains stable. The existence of a subset of PMN which spontaneously expresses PR3 and varies among individuals, may be relevant to the pathogenesis of anti-PR3 ANCA autoantibody-related vasculitis.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>7589506</pmid><doi>10.1016/0014-5793(95)01073-N</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | ANCA ANCA, antineutrophil cytoplasmic autoantibodies Animals Arthritis, Rheumatoid - immunology Autoantibodies - immunology Blood Donors Cell Membrane - enzymology cyt. B, cytochalasin B fMLP, N-formyl-methionyl-leucyl-phenylalanine Granulomatosis with Polyangiitis - immunology HBSS, Hanks' balanced salt solution Humans Mice Myeloblastin N-Formylmethionine Leucyl-Phenylalanine Neutrophils - enzymology Neutrophils - immunology Phenotype PMN serine protease PMN, polymorphonuclear neutrophils Polymorphonuclear neutrophil PR3, proteinase 3 Proteinase 3 Serine Endopeptidases - classification Serine Endopeptidases - metabolism Vasculitis WG, Wegener's granulomatosis |
title | Bimodal distribution of proteinase 3 (PR3) surface expression reflects a constitutive heterogeneity in the polymorphonuclear neutrophil pool |
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