Cerebrovascular permeability to horseradish peroxidase in hypertensive rats: effects of unilateral locus ceruleus lesion

Unilateral locus ceruleus lesion enhances leakage of radioiodinated human serum albumin into the ipsilateral cerebral cortex of rats with norepinephrine-induced hypertension. This ultrastructural study was undertaken, to determine the mechanism by which this permeability alteration occurs, using hor...

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Bibliographic Details
Published in:Acta neuropathologica 1987-01, Vol.73 (3), p.247-253
Main Authors: NAG, S, HARIK, S. I
Format: Article
Language:English
Subjects:
Animals
Arterial hypertension. Arterial hypotension
Arterioles - ultrastructure
Biological and medical sciences
Blood and lymphatic vessels
Blood Pressure
Blood-Brain Barrier
Brain - blood supply
Brain - metabolism
Brain - ultrastructure
Capillary Permeability
Cardiology. Vascular system
Experimental diseases
Horseradish Peroxidase - metabolism
Hydroxydopamines
Hypertension - metabolism
Hypertension - pathology
Locus Coeruleus - physiology
Male
Medical sciences
Microscopy, Electron
Oxidopamine
Peroxidases - metabolism
Rats
Rats, Inbred WF
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Summary:Unilateral locus ceruleus lesion enhances leakage of radioiodinated human serum albumin into the ipsilateral cerebral cortex of rats with norepinephrine-induced hypertension. This ultrastructural study was undertaken, to determine the mechanism by which this permeability alteration occurs, using horseradish peroxidase (HRP) as a tracer. Unilateral locus ceruleus lesion was produced in male Wistar-Furth rats by stereotaxic microinfusion of 5 micrograms of 6-hydroxydopamine. Two weeks later, rats were injected with HRP intravenously and acute hypertension was induced in awake rats by an intravenous infusion of norepinephrine (6 micrograms), epinephrine (6 micrograms) or angiotensin amide (12 micrograms) given over a 2-min period. Thirty seconds later, the rats were perfused with fixative under deep anesthesia and their brains were sliced and processed for demonstration of HRP reaction product. Leakage of HRP occurred in both cerebral hemispheres in response to hypertension induced by the three pressor agents, but the leakage was greater on the lesioned side in response to epinephrine and norepinephrine, while in the case of angiotensin-induced hypertension side-to-side differences in permeability alterations were not observed. In both cerebral hemispheres increased permeability affected mainly arterioles, which showed enhanced pinocytosis as the principal mechanism of HRP extravasation.
ISSN:0001-6322
1432-0533
DOI:10.1007/BF00686618