Listeria monocytogenes infection increases neutrophil adhesion and damage to a murine hepatocyte cell line in vitro

• Published in: Immunology letters 1995-05, Vol.46 (1), p.111-116
• Main Authors: Boury, Nancy Maroushek, Czuprynski, Charles J.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - pharmacology; Cell Adhesion - immunology; Cell Line; Hepatocyte; ICAM-1; Immunosuppressive Agents - pharmacology; Intercellular Adhesion Molecule-1 - immunology; Listeria monocytogenes; Listeria monocytogenes - genetics; Listeria monocytogenes - immunology; Listeriosis - immunology; Liver - cytology; Liver - immunology; Liver - microbiology; Macrophage-1 Antigen - immunology; Mice; Neutrophil; Neutrophils - immunology; Neutrophils - microbiology; Oxidative Stress - immunology
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/0165-2478(95)00027-3

Several studies have reported that Listeria monocytogenes multiplies within hepatocytes and that inflammatory neutrophils inhibit this intracellular growth in vivo. In the present study, we used a murine embryonic hepatocyte cell line (ATCC TIB73) as an in vitro model to investigate neutrophil-hepatocyte interactions. Murine peritoneal exudate neutrophils adhered more readily to L. monocytogenes-infected hepatocyte monolayers than to uninfected monolayers or monolayers infected with actA − and hly − mutants of L. monocytogenes. L. monocytogenes-infected TIB73 cells increased their surface expression of ICAM-1 as compared with uninfected TIB73 cells. Neutrophil adherence and oxidative stress to TIB73 cells were reduced by pre-incubating the hepatocyte monolayers with anti-ICAM-1 monoclonal antibody and diminished further by pre-incubating the peritoneal exudate neutrophils with an anti-CR3 monoclonal antibody.