Fine structure of tooth germs during the formation of enameloid matrix in Tilapia nilotica, a teleost fish

• Published in: Archives of oral biology 1995-09, Vol.40 (9), p.801-814
• Main Author: Sasagawa, I.
• Format: Article
• Language: English
• Subjects: Amelogenesis; Animals; Cell Nucleus - ultrastructure; Collagen - ultrastructure; collagen fibrils; Coloring Agents; Cytoplasmic Granules - ultrastructure; Dental Enamel - embryology; Dental Enamel - ultrastructure; Dentistry; enameloid; Endoplasmic Reticulum, Rough - ultrastructure; Epithelium - embryology; Epithelium - ultrastructure; fine structure; Glycosaminoglycans - ultrastructure; Golgi Apparatus - ultrastructure; Microscopy, Electron; Microtubules - ultrastructure; Mitochondria - ultrastructure; Odontoblasts - ultrastructure; Phosphotungstic Acid; Procollagen - ultrastructure; Proteoglycans - ultrastructure; teleost; Tilapia - embryology; tooth development; Tooth Germ - ultrastructure; Vacuoles - ultrastructure
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/0003-9969(95)00050-Y

Tooth germs were examined by light and transmission electron microscopy. Collagen fibrils were relatively dispersed and thin at the early and middle stages of formation of the enameloid matrix, when the enameloid layer was thin. At the late stage, the fibrils became thicker, reaching nearly 30 nm dia, and formed the interwoven thick bundles that are characteristic of teleost cap enameloid. Abundant flocculent and/or fine, network-like material, probably representing glycosaminoglycans or proteoglycans, was located between the collagen fibrils. Tall, columnar, inner dental epithelial cells contained abundant rough endoplasmic reticulum and many mitochondria, and a well-developed Golgi apparatus was seen around the nuclei at the late stage. Elongated vesicles enclosing fine, filamentous material that resembled procollagen granules, and large granules containing fibril-like structures that were 150 nm in thickness and had periodic cross-banding at 32-nm intervals, were usually observed near the Golgi apparatus. The contents of the large granules were well stained with phosphotungstic acid, which suggests that inner dental epithelial cells synthesize collagen fibrils. At this time, odontoblasts also contained abundant rough endoplasmic reticulum and mitochondria, a well-developed Golgi, several kinds of granule including those that probably contained procollagen, and many microtubules. It is proposed that odontoblasts are involved in the formation of a considerable portion of the enameloid matrix, including collagen fibrils.