Transfer of brain dopamine system-specific quantitative trait loci onto a C57BL/6ByJ background

Analysis of complex traits has been difficult because they are usually affected by multiple genes, by the environment, and, importantly, by intra-locus, intergenic, and genotype-environment interactions. To eliminate some of these obstacles and thus provide a tool for identifying specific gene effec...

Full description

Saved in:
Bibliographic Details
Published in:Mammalian genome 1994-11, Vol.5 (11), p.735-737
Main Authors: Vadász, C, Sziráki, I, Murthy, L R, Sasvári-Székely, M, Kabai, P, Laszlovszky, I, Fleischer, A, Juhász, B, Zahorchak, R
Format: Article
Language:English
Subjects:
Animals
Cell Count
Chromosome Mapping
Crosses, Genetic
Dopamine - biosynthesis
Exploratory Behavior
Female
Gene Expression Regulation
Genetic Markers
Male
Mesencephalon - cytology
Mesencephalon - enzymology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL - genetics
Mice, Inbred C57BL - metabolism
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neurons - enzymology
Species Specificity
Tyrosine 3-Monooxygenase - genetics
Tyrosine 3-Monooxygenase - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Analysis of complex traits has been difficult because they are usually affected by multiple genes, by the environment, and, importantly, by intra-locus, intergenic, and genotype-environment interactions. To eliminate some of these obstacles and thus provide a tool for identifying specific gene effects in a complex trait, we conceptualized and developed quantitative trait loci (QTL) Introgression (QI) lines and derived Recombinant QTL Introgression (RQI) strains from the QI lines. In genetic background the QI lines are nearly identical; however, differences at one or a few loci are responsible for differences in the phenotype of interest. In addition, QI lines carry passenger genes from the donor genome, which are linked to the differential gene and reside on the same chromosome segment, and whose presence makes chromosome mapping of the differential genes feasible. The mesencephalic activity of tyrosine hydroxylase (TH/MES) was our target phenotype, because it demonstrates significant genetic variation, because the differences in TH/MES can be attributed entirely to differences in number of dopamine (DA) neurons in the midbrain, and because DA systems affect many aspects of behavior. Here we report the development of replicated QI lines with significantly different TH/MES and our preliminary results on behavioral consequences of genetic manipulation of the mesotelencephalic DA system.
ISSN:0938-8990
1432-1777
DOI:10.1007/BF00426084