Infectious Proviral Clones of Chimpanzee Foamy Virus (SFVcpz) Generated by Long PCR Reveal Close Functional Relatedness to Human Foamy Virus

Infectious proviral clones of simian foamy virus isolated from chimpanzee (SFVcpz) were generated by long PCR. Two overlapping fragments representing the complete provirus were amplified from genomic DNA of infected cells. Four 8.8-kbp amplimers extending from base 1 of the provirus into theenvgene...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1995-12, Vol.214 (2), p.685-689
Main Authors: HERCHENRÖDER, OTTMAR, TUREK, ROBERT, NEUMANN-HAEFELIN, DIETER, RETHWILM, AXEL, SCHNEIDER, JOSEF
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Line
DNA Primers
DNA, Viral
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
foamy virus
Humans
Molecular Sequence Data
Pan troglodytes - virology
Polymerase Chain Reaction - methods
Promoter Regions, Genetic
Proviruses - genetics
Proviruses - pathogenicity
Repetitive Sequences, Nucleic Acid
Retroviridae Proteins - genetics
Retroviridae Proteins - metabolism
Spumavirus - classification
Spumavirus - genetics
Spumavirus - pathogenicity
Trans-Activators - genetics
Trans-Activators - metabolism
Transcriptional Activation
Virus Latency
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Summary:Infectious proviral clones of simian foamy virus isolated from chimpanzee (SFVcpz) were generated by long PCR. Two overlapping fragments representing the complete provirus were amplified from genomic DNA of infected cells. Four 8.8-kbp amplimers extending from base 1 of the provirus into theenvgene and five 4.45-kbp amplimers reaching fromenvto the end of the 3′-LTR were cloned into pCR II. Subsequently, the proviral fragments were combined in a chessboard manner to generate 20 plasmids containing full-length proviral DNA. Four plasmids produced infectious virus after transfection of susceptible cells. A distinct proviral form bearing a deletion in the transactivator gene joining both exons of a second regulatory gene present in wild-type foamy virus-infected cells started to emerge 48 hr after transfection of BHK cells with infectious SFVcpz DNA. This observation supports a novel hypothesis to explain establishment of foamy virus latency. The transactivator protein Taf of SFVcpz transcomplemented for the homologous protein Bel-1 of the unique human foamy virus isolate (HFV) and Bel-1 exhibited the reciprocal activity, suggesting that HFV could represent a variant of chimpanzee foamy virus.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1995.0086